caa a22 4500 445888954 CHVBK 20180317145609.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00535-010-0369-3 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0369-3 Identification of a high molecular weight kininogen fragment as a marker for early gastric cancer by serum proteome analysis [Elektronische Daten] [Hiroshi Umemura, Akira Togawa, Kazuyuki Sogawa, Mamoru Satoh, Kaoru Mogushi, Motoi Nishimura, Kazuyuki Matsushita, Hiroshi Tanaka, Hirotaka Takizawa, Yoshio Kodera, Fumio Nomura] Background: Serum biomarkers currently available for gastric cancers are not sufficiently sensitive and specific. Methods: We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) to generate comparative peptide profiles of serum samples obtained from gastric cancer patients (n=81) and age- and sex-matched healthy controls (n=66). Results: Because of initial screening and further validation, we found that the intensities of a 2209 m/z MS peak were increased in the preoperative sera obtained from gastric cancer patients, and we identified this peak, a 2209Da peptide, as a high molecular weight (HMW) kininogen fragment. Receiver operating characteristic analyses showed that the area under the curve (AUC) for the 2209Da peptide (AUC=0.715) was greater than those for conventional tumor markers (carcinoembryonic antigen AUC=0.593, carbohydrate antigen 19-9 AUC=0.527) used for the detection of stage I gastric cancers. Inverse correlations were observed between the levels of intact HMW kininogen and the 2209Da peptide, suggesting that the upregulation of some protease activities is responsible for the overproduction of a kininogen fragment in gastric cancer patients. Conclusions: Serum levels of the 2209Da peptide identified in this study have a greater diagnostic ability than those of conventional tumor markers used for the early detection of gastric cancer. Springer, 2011 Gastric adenocarcinoma nationallicence MALDI-TOF MS nationallicence Proteomics nationallicence Serum biomarkers nationallicence High molecular weight kininogen nationallicence Umemura Hiroshi Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut Togawa Akira Department of Surgery, Kimitsu Chuo Hospital, Kisarazu, Japan aut Sogawa Kazuyuki Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut Satoh Mamoru Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut Mogushi Kaoru Information Center for Medical Sciences, Tokyo Medical and Dental University, Tokyo, Japan aut Nishimura Motoi Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut Matsushita Kazuyuki Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut Tanaka Hiroshi Information Center for Medical Sciences, Tokyo Medical and Dental University, Tokyo, Japan aut Takizawa Hirotaka Kashiwado Memorial Foundation, PortSquare Kashiwado Clinic, Chiba, Japan aut Kodera Yoshio Laboratory of Biomolecular Dynamics, Department of Physics, School of Science, Kitasato University, Sagamihara, Japan aut Nomura Fumio Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut Journal of Gastroenterology Springer Japan 46/5(2011-05-01), 577-585 0944-1174 46:5<577 2011 46 535 https://doi.org/10.1007/s00535-010-0369-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0369-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Umemura Hiroshi Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut NATIONALLICENCE 700 1- Togawa Akira Department of Surgery, Kimitsu Chuo Hospital, Kisarazu, Japan aut NATIONALLICENCE 700 1- Sogawa Kazuyuki Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut NATIONALLICENCE 700 1- Satoh Mamoru Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut NATIONALLICENCE 700 1- Mogushi Kaoru Information Center for Medical Sciences, Tokyo Medical and Dental University, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishimura Motoi Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut NATIONALLICENCE 700 1- Matsushita Kazuyuki Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut NATIONALLICENCE 700 1- Tanaka Hiroshi Information Center for Medical Sciences, Tokyo Medical and Dental University, Tokyo, Japan aut NATIONALLICENCE 700 1- Takizawa Hirotaka Kashiwado Memorial Foundation, PortSquare Kashiwado Clinic, Chiba, Japan aut NATIONALLICENCE 700 1- Kodera Yoshio Laboratory of Biomolecular Dynamics, Department of Physics, School of Science, Kitasato University, Sagamihara, Japan aut NATIONALLICENCE 700 1- Nomura Fumio Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8670, Chiba, Chiba, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/5(2011-05-01), 577-585 0944-1174 46:5<577 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer