caa a22 4500 445889004 CHVBK 20180317145609.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00535-010-0330-5 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0330-5 Development of an experimental model of cholestasis induced by hypoxic/ischemic damage to the bile duct and liver tissues in infantile rats [Elektronische Daten] [Fumiaki Toki, Atsushi Takahashi, Makoto Suzuki, Sayaka Ootake, Junko Hirato, Hiroyuki Kuwano] Background: We aimed to develop experimental models of hypoxia/ischemia-induced cholestasis using neonatal and infantile rats. Methods: Hypoxia/ischemia was induced in the bile duct (BD) by injecting prostaglandin (PG) at birth and/or by coagulation of the hepatic artery (CHA) at about 3weeks after birth. The rats were divided into 6 groups: control; PG-injected; sham-operated with or without PG; CHA; and CHA+PG. CHA was also performed in adult rats. Liver specimens and blood samples were obtained at 5weeks after birth, and immunohistochemical and biochemical examinations were performed. Results: (1) BD proliferation with fibrosis (BDPF) was found in the intrahepatic portal tract in the CHA and CHA+PG groups. Low-grade BDPF was observed in the PG group. (2) Cyst formation in the extrahepatic BD (EBD) was observed in the porta hepatis of some rats in the CHA and CHA+PG groups. In these groups, the number of peribiliary vascular plexuses (PVPs) decreased. BD proliferation and infiltration of inflammatory cells were observed in the EBD wall in the CHA+PG group. (3) Ki-67 was expressed in BD and EBD cells in the CHA+PG group. (4) BDPF was not detected in adult rats with CHA. (5) Serum liver function tests indicated obstructive changes in the EBD in the CHA and CHA+PG groups. Conclusion: Reduced blood flow in the EBD during infancy induced BDPF and obstructive changes in the EBD, which may, along with immature PVP and inflammatory changes in the EBD, contribute to hypoxia/ischemia of the EBD. Springer, 2011 Bile duct proliferation with fibrosis nationallicence Cholestasis nationallicence Hypoxia/ischemia nationallicence Inflammation nationallicence Neonate nationallicence Infant nationallicence Toki Fumiaki Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut Takahashi Atsushi Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut Suzuki Makoto Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut Ootake Sayaka Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut Hirato Junko Department of Pathology, Gunma University Hospital, Maebashi, Japan aut Kuwano Hiroyuki Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut Journal of Gastroenterology Springer Japan 46/5(2011-05-01), 639-647 0944-1174 46:5<639 2011 46 535 https://doi.org/10.1007/s00535-010-0330-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0330-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toki Fumiaki Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Takahashi Atsushi Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Suzuki Makoto Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Ootake Sayaka Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Hirato Junko Department of Pathology, Gunma University Hospital, Maebashi, Japan aut NATIONALLICENCE 700 1- Kuwano Hiroyuki Pediatric Surgical Unit, Department of General Surgical Science, Gunma University Hospital, 3-39-15 Showa-machi, 371-8511, Maebashi, Gunma, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/5(2011-05-01), 639-647 0944-1174 46:5<639 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer