caa a22 4500 445889152 CHVBK 20180317145610.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00535-010-0318-1 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0318-1 DPEP1, expressed in the early stages of colon carcinogenesis, affects cancer cell invasiveness [Elektronische Daten] [Yuji Toiyama, Yasuhiro Inoue, Hiromi Yasuda, Susumu Saigusa, Takeshi Yokoe, Yoshinaga Okugawa, Koji Tanaka, Chikao Miki, Masato Kusunoki] Background: We investigated changes in the gene expression profile in colon cancer in order to identify gene markers that may be useful in the management of this disease. Methods: The Cancer Genome Anatomy Project was used to detect differences in gene expression between normal and cancer tissue. The overexpression of dipeptidase-1 (DPEP1) in cancer tissue was confirmed in a sample of 76 patients by real-time PCR. To identify the function of DPEP1, RNA interference (RNAi) was used to inactivate this gene in the colon cancer cell line. Immunohistochemical analysis was performed to characterize the pattern of DPEP1 expression in colon cancer. Results: DPEP1 expression in cancer was significantly higher than that in normal tissue. However, DPEP1 expression decreased with pathological differentiation, lymph-node and distant metastasis. Patients with tumors with decreased DPEP1 expression showed a poorer prognosis, and this was also true of patients with tumors who are treated with curative intent. RNAi-mediated DPEP1 reduction in the colon cancer cell line did not result in cell proliferation or apoptosis, but was associated with an increased invasive ability. DPEP1 protein was observed on the apical side of the cancer cells, and is expressed in the early stages of carcinogenesis, even in adenomas of both sporadic colorectal cancer and familial adenomatous polyposis patients. Conclusions: DPEP1 expression in normal colonic mucosa is very low, but it is highly expressed in colorectal adenoma and cancer specimens and is negatively correlated with parameters of pathological aggressiveness and poor prognosis. DPEP1 is expressed in the early stages of colon carcinogenesis and affects cancer cell invasiveness. Springer, 2010 DPEP1 nationallicence Colorectal cancer nationallicence Prognosis nationallicence Cancer invasion nationallicence Carcinogenesis nationallicence Toiyama Yuji Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Inoue Yasuhiro Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Yasuda Hiromi Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Saigusa Susumu Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Yokoe Takeshi Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Okugawa Yoshinaga Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Tanaka Koji Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Miki Chikao Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Kusunoki Masato Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut Journal of Gastroenterology Springer Japan 46/2(2011-02-01), 153-163 0944-1174 46:2<153 2011 46 535 https://doi.org/10.1007/s00535-010-0318-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0318-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toiyama Yuji Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Inoue Yasuhiro Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Yasuda Hiromi Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Saigusa Susumu Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Yokoe Takeshi Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Okugawa Yoshinaga Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Tanaka Koji Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Miki Chikao Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 700 1- Kusunoki Masato Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, 514-8507, Tsu, Mie, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/2(2011-02-01), 153-163 0944-1174 46:2<153 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer