caa a22 4500 445889160 CHVBK 20180317145610.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00535-010-0296-3 doi (NATIONALLICENCE)springer-10.1007/s00535-010-0296-3 Overexpression of apelin receptor (APJ/AGTRL1) on hepatic stellate cells and sinusoidal angiogenesis in human cirrhotic liver [Elektronische Daten] [Hiroaki Yokomori, Masaya Oda, Kazunori Yoshimura, Sanae Machida, Fumihiko Kaneko, Toshifumi Hibi] Background: The apelin receptor (APJ) is related to angiotensin-like-receptor 1 (AGTRL1). This study was designed to elucidate the in vivo localization and changes of APJ in cirrhotic liver, and the in vitro changes of APJ expression in cultured hepatic stellate cells (HSCs) and capillarized sinusoidal endothelial cells (SECs) activated by growth factors. Methods: In vivo studies used control liver samples, cirrhotic liver samples from patients with Child's A cirrhosis undergoing surgical resection (Child-A-LC), and cirrhotic liver samples from autopsied cases of decompensated Child's C cirrhosis (Child-C-LC). Immunohistochemical (IHC), Western blot, laser-capture microdissection (LCM) coupled with reverse transcription -polymerase chain reaction (RT-PCR), and immunoelectron microscopic (IEM) studies for APJ expression were conducted. In vitro examinations used commercial human HSCs and SECs. APJ expression was examined in cultured HSCs activated by growth factors and in capillarized SECs activated by angiogenic factors. Results: The IHC study of liver samples revealed only slight APJ expression in hepatic sinusoids in control liver tissue. In cirrhotic liver (Child-A-LC and Child-C-LC), APJ expression was evident mainly along the sinusoids and on portal fibroblasts in fibrotic septa. Western blot analysis of whole-liver homogenate and LCM-PCR of sinusoids revealed overexpression of APJ in Child-C-LC samples. The results of IEM studies showed that APJ expression was increased significantly on HSCs, but it was sparse on SECs in Child-C-LC tissue. In vitro examination revealed that APJ was overexpressed in cultured HSCs activated by platelet-derived growth factor-β. Conclusions: Enhanced expression of APJ on HSCs in cirrhosis indicates markedly increased vascular remodeling. Springer, 2010 Liver cirrhosis nationallicence Apelin receptor nationallicence Vascular remodeling nationallicence Yokomori Hiroaki Department of Internal Medicine, Kitasato Institute Medical Center Hospital, Kitasato University, 6-100 Arai, 364-8501, Kitamoto, Saitama, Japan aut Oda Masaya Organized Center of Clinical Medicine, International University of Health and Welfare, Tokyo, Japan aut Yoshimura Kazunori Department of Rehabilitation, Nihon Institute of Medical Science, Saitama, Japan aut Machida Sanae Biomedical Research Center, Saitama Medical University, Saitama, Japan aut Kaneko Fumihiko Department of Internal Medicine, Kitasato Institute Medical Center Hospital, Kitasato University, 6-100 Arai, 364-8501, Kitamoto, Saitama, Japan aut Hibi Toshifumi Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan aut Journal of Gastroenterology Springer Japan 46/2(2011-02-01), 222-231 0944-1174 46:2<222 2011 46 535 https://doi.org/10.1007/s00535-010-0296-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00535-010-0296-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yokomori Hiroaki Department of Internal Medicine, Kitasato Institute Medical Center Hospital, Kitasato University, 6-100 Arai, 364-8501, Kitamoto, Saitama, Japan aut NATIONALLICENCE 700 1- Oda Masaya Organized Center of Clinical Medicine, International University of Health and Welfare, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshimura Kazunori Department of Rehabilitation, Nihon Institute of Medical Science, Saitama, Japan aut NATIONALLICENCE 700 1- Machida Sanae Biomedical Research Center, Saitama Medical University, Saitama, Japan aut NATIONALLICENCE 700 1- Kaneko Fumihiko Department of Internal Medicine, Kitasato Institute Medical Center Hospital, Kitasato University, 6-100 Arai, 364-8501, Kitamoto, Saitama, Japan aut NATIONALLICENCE 700 1- Hibi Toshifumi Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Gastroenterology Springer Japan 46/2(2011-02-01), 222-231 0944-1174 46:2<222 2011 46 535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer