caa a22 4500 445889373 CHVBK 20180317145611.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-010-9618-9 doi (NATIONALLICENCE)springer-10.1007/s11120-010-9618-9 Acclimation to low or limiting CO2 in non-synchronous Chlamydomonas causes a transient synchronization of the cell division cycle [Elektronische Daten] [Steven Dillard, Kyujung Van, Martin Spalding] Acclimation of Chlamydomonas reinhardtii (hereafter, Chlamydomonas) to low or limiting CO2 or inorganic carbon (Ci) has been studied fairly extensively with regard to the mechanisms underlying the inducible Ci acquisition systems and the signal transduction pathway involved in recognizing and responding to decreased Ci availability. Investigation of low Ci acclimation responses typically is performed with non-synchronous cultures grown in continuous light to avoid any effects of the cell division cycle (CDC) confounding interpretation of acclimation responses. However, little is known about whether acclimation to low Ci might affect the distribution of cells among the various stages of the CDC. To investigate the effects of a limiting-Ci challenge on the CDC of Chlamydomonas, flow cytometry was used to monitor the distribution of cells among the CDC stages in both synchronous and non-synchronous cultures during acclimation to low or limiting Ci. When faced with Ci limitation, non-synchronous cultures of Chlamydomonas undergo transient synchronization as those cells past the Commitment point of the CDC undergo division, while the remainder of the cells pause their growth in early G-phase, with the result that the cells all accumulate in early G-phase, appearing transiently synchronized until acclimated sufficiently to the decreased Ci for growth to resume. This perturbation of the CDC by a limiting-Ci challenge has important implications for the interpretation of gene expression and other responses apparently induced by low or limiting Ci. Springer Science+Business Media B.V., 2011 CO2-concentrating mechanism nationallicence CCM nationallicence Chlamydomonas reinhardtii nationallicence CAH1 nationallicence Bicarbonate nationallicence Inorganic carbon nationallicence CDC nationallicence Dillard Steven Biology Program, Iowa State University, 50011, Ames, IA, USA aut Van Kyujung Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, South Korea aut Spalding Martin Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 161-168 0166-8595 109:1-3<161 2011 109 11120 https://doi.org/10.1007/s11120-010-9618-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-010-9618-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dillard Steven Biology Program, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Van Kyujung Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, 151-921, Seoul, South Korea aut NATIONALLICENCE 700 1- Spalding Martin Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 161-168 0166-8595 109:1-3<161 2011 109 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer