caa a22 4500 445889438 CHVBK 20180317145611.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9622-8 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9622-8 High-throughput pyrosequencing of the chloroplast genome of a highly neutral-lipid-producing marine pennate diatom, Fistulifera sp. strain JPCC DA0580 [Elektronische Daten] [Tsuyoshi Tanaka, Yorikane Fukuda, Tomoko Yoshino, Yoshiaki Maeda, Masaki Muto, Mitsufumi Matsumoto, Shigeki Mayama, Tadashi Matsunaga] The chloroplast genome of the highly neutral-lipid-producing marine pennate diatom Fistulifera sp. strain JPCC DA0580 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918bp with an inverted repeat of 13,330bp and is slightly larger than the other diatom chloroplast genomes due to several low gene-density regions lacking similarity to the other diatom chloroplast genomes. Protein-coding genes were nearly identical to those from Phaeodactylum tricornutum. On the other hand, we found unique sequence variations in genes of photosystem II which differ from the consensus in other diatom chloroplasts. Furthermore, five functional unknown ORFs and a putative serine recombinase gene, serC2, are located in the low gene-density regions. SerC2 was also identified in the plasmids of another pennate diatom, Cylindrotheca fusiformis, and in the plastid genome of the diatom endosymbiont of Kryptoperidinium foliaceum. Exogenous plasmids might have been incorporated into the chloroplast genome of Fistulifera sp. by lateral gene transfer. Chloroplast genome sequencing analysis of this novel diatom provides many important insights into diatom evolution. Springer Science+Business Media B.V., 2011 Diatom nationallicence Chloroplast genome nationallicence Next-generation DNA sequencing nationallicence Lateral gene transfer nationallicence Endosymbiosis nationallicence Tanaka Tsuyoshi Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut Fukuda Yorikane The Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut Yoshino Tomoko Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut Maeda Yoshiaki Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut Muto Masaki The Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut Matsumoto Mitsufumi Biotechnology Laboratory, Electric Power Development Co., Ltd, 1, Yanagisaki-machi, 808-0111, Wakamatsu-ku, Kitakyusyu, Japan aut Mayama Shigeki Department of Biology, Tokyo Gakugei University, 4-1-1, Nukuikita-machi, Koganei, 184-8501, Tokyo, Japan aut Matsunaga Tadashi Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 223-229 0166-8595 109:1-3<223 2011 109 11120 https://doi.org/10.1007/s11120-011-9622-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9622-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Tsuyoshi Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut NATIONALLICENCE 700 1- Fukuda Yorikane The Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshino Tomoko Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut NATIONALLICENCE 700 1- Maeda Yoshiaki Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut NATIONALLICENCE 700 1- Muto Masaki The Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsumoto Mitsufumi Biotechnology Laboratory, Electric Power Development Co., Ltd, 1, Yanagisaki-machi, 808-0111, Wakamatsu-ku, Kitakyusyu, Japan aut NATIONALLICENCE 700 1- Mayama Shigeki Department of Biology, Tokyo Gakugei University, 4-1-1, Nukuikita-machi, Koganei, 184-8501, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsunaga Tadashi Department of Biotechnology, Faculty of Engineering, Tokyo University of Agriculture & Technology, 2-24-16, Naka-cho, Koganei, 184-8588, Tokyo, Japan aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 223-229 0166-8595 109:1-3<223 2011 109 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer