caa a22 4500 445889500 CHVBK 20180317145611.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-010-9607-z doi (NATIONALLICENCE)springer-10.1007/s11120-010-9607-z Spijkerman Elly Department of Ecology and Ecosystem Modelling, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany aut The expression of a carbon concentrating mechanism in Chlamydomonas acidophila under variable phosphorus, iron, and CO2 concentrations [Elektronische Daten] [Elly Spijkerman] The CO2 acquisition was analyzed in Chlamydomonas acidophila at pH 2.4 in a range of medium P and Fe concentrations and at high and low CO2 condition. The inorganic carbon concentrating factor (CCF) was related to cellular P quota (Q p), maximum CO2-uptake rate by photosynthesis ($$ V_{{{ \max },{\text{O}}_{ 2} }} $$), half saturation constant for CO2 uptake (K 0.5), and medium Fe concentration. There was no effect of the medium Fe concentration on the CCF. The CCF increased with increasing Q p in both high and low CO2 grown algae, but maximum Q p was 6-fold higher in the low CO2 cells. In high CO2 conditions, the CCF was low, ranging between 0.8 and 3.5. High CCF values up to 9.1 were only observed in CO2-limited cells, but P- and CO2-colimited cells had a low CCF. High CCF did not relate with a low K 0.5 as all CO2-limited cells had a low K 0.5 (<4μM CO2). High Ci-pools in cells with high Q p suggested the presence of an active CO2-uptake mechanism. The CCF also increased with increasing $$ V_{{{ \max },{\text{O}}_{ 2} }} $$ which reflect an adaptation to the nutrient in highest demand (CO2) under balanced growth conditions. It is proposed that the size of the CCF in C. acidophila is more strongly related to porter density for CO2 uptake (reflected in $$ V_{{{ \max },{\text{O}}_{ 2} }} $$) and less- to high-affinity CO2 uptake (low K 0.5) at balanced growth. In addition, high CCF can only be realized with high Q p. Springer Science+Business Media B.V., 2011 C3 photosynthesis nationallicence Micro-algae nationallicence Carbon concentrating mechanism nationallicence Phosphorus limitation nationallicence Iron toxicity nationallicence α : Initial slope of the P-E curve nationallicence CCF : Ci concentrating factor nationallicence CCM : Ci concentrating mechanism nationallicence Chl a : Chlorophyll a nationallicence Ci : Inorganic carbon nationallicence Ci-pool : concentration of Ci in the cell nationallicence E : Light irradiance nationallicence K 0.5 : Half saturation constant for CO2 uptake nationallicence P max : Gross maximum photosynthetic rate nationallicence Q p : Cellular P quota nationallicence R d : Dark respiration rate nationallicence V max,C : Maximum CO2 uptake rate measured by C-fixation nationallicence $$ V_{{{ \max },{\text{O}}_{ 2} }} $$ : Maximum CO2 uptake rate measured by photosynthesis nationallicence Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 179-189 0166-8595 109:1-3<179 2011 109 11120 https://doi.org/10.1007/s11120-010-9607-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-010-9607-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Spijkerman Elly Department of Ecology and Ecosystem Modelling, University of Potsdam, Am Neuen Palais 10, 14469, Potsdam, Germany aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 179-189 0166-8595 109:1-3<179 2011 109 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer