caa a22 4500 445889586 CHVBK 20180317145611.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9635-3 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9635-3 The carbonic anhydrase isoforms of Chlamydomonas reinhardtii [Elektronische Daten] intracellular location, expression, and physiological roles [James Moroney, Yunbing Ma, Wesley Frey, Katelyn Fusilier, Trang Pham, Tiffany Simms, Robert DiMario, Jing Yang, Bratati Mukherjee] Aquatic photosynthetic organisms, such as the green alga Chlamydomonas reinhardtii, respond to low CO2 conditions by inducing a CO2 concentrating mechanism (CCM). Carbonic anhydrases (CAs) are important components of the CCM. CAs are zinc-containing metalloenzymes that catalyze the reversible interconversion of CO2 and HCO3 −. In C. reinhardtii, there are at least 12 genes that encode CA isoforms, including three alpha, six beta, and three gamma or gamma-like CAs. The expression of the three alpha and six beta genes has been measured from cells grown on elevated CO2 (having no active CCM) versus cells growing on low levels of CO2 (with an active CCM) using northern blots, differential hybridization to DNA chips and quantitative RT-PCR. Recent RNA-seq profiles add to our knowledge of the expression of all of the CA genes. In addition, protein content for some of the CA isoforms was estimated using antibodies corresponding to the specific CA isoforms: CAH1/2, CAH3, CAH4/5, CAH6, and CAH7. The intracellular location of each of the CA isoforms was elucidated using immunolocalization and cell fractionation techniques. Combining these results with previous studies using CA mutant strains, we will discuss possible physiological roles of the CA isoforms concentrating on how these CAs might contribute to the acquisition and retention of CO2 in C. reinhardtii. Springer Science+Business Media B.V., 2011 Carbonic anhydrase nationallicence CO2 concentrating mechanism nationallicence CCM nationallicence Chlamydomonas nationallicence Pyrenoid nationallicence Moroney James Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Ma Yunbing Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Frey Wesley Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Fusilier Katelyn Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Pham Trang Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Simms Tiffany Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut DiMario Robert Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Yang Jing Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Mukherjee Bratati Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 133-149 0166-8595 109:1-3<133 2011 109 11120 https://doi.org/10.1007/s11120-011-9635-3 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9635-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moroney James Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Ma Yunbing Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Frey Wesley Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Fusilier Katelyn Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Pham Trang Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Simms Tiffany Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- DiMario Robert Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Yang Jing Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Mukherjee Bratati Department of Biological Sciences, Louisiana State University, 70803, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 133-149 0166-8595 109:1-3<133 2011 109 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer