caa a22 4500 445889594 CHVBK 20180317145611.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-010-9619-8 doi (NATIONALLICENCE)springer-10.1007/s11120-010-9619-8 Regulation of the expression of H43/Fea1 by multi-signals [Elektronische Daten] [Masato Baba, Yutaka Hanawa, Iwane Suzuki, Yoshihiro Shiraiwa] The composition of extracellular proteins is known to be drastically changed in the unicellular green alga Chlamydomonas reinhardtii when the cells are transferred from ambient CO2 to elevated CO2 conditions. We previously observed very high production of the H43/Fea1 protein under high-CO2 (0.3-3% in air) conditions. In addition, H43/Fea1 gene expression was reported to be induced under iron-deficient and cadmium-excess conditions, but it remains unclear how gene expression is regulated by multiple signals. To elucidate the regulatory mechanism of H43/Fea1 expression, this study intended to identify a high-CO2-responsive cis-element in a wall-deficient strain C. reinhardtti CC-400. Cells incubated in the presence of acetate in the dark, namely heterotrophically generated high-CO2 conditions, were used for inducing H43/Fea1 gene expression following our previous study (Hanawa et al., Plant Cell Physiol 48:299-309, 2007) in Fe-sufficient and Cd-deficient medium to prevent the generation of other signals. First, we constructed a reporter assay system using transformants constructed by introducing genes with series of 5′-deleted upstream sequences of H43/Fea1 that were fused to a coding sequence of the Ars for arylsulfatase2 reporter gene. Consequently, the high-CO2-responsive cis-element (HCRE) was found to be located at a −537/−370 upstream region from the transcriptional initiation site of H43/Fea1. However, it still remains possible that a −724/−537 upstream region may also have a significant role in activating gene expression regulated by high-CO2. Remarkably, a −925/−370 upstream region could successfully activate the Ars reporter gene under heterotrophically generated high-CO2 conditions even when the sequence containing two Fe-deficiency-responsive elements was completely deleted. These results clearly showed that H43/Fea1 expression is regulated by high-CO2 signal independently via the HCRE that is located distantly from Fe-deficient-signal responsive element, indicating that H43/Fea1 is a multi-signal-regulated gene. Springer Science+Business Media B.V., 2011 Chlamydomonas reinhardtii nationallicence cis -Element nationallicence Gene expression nationallicence H43/Fea1 nationallicence High-CO2 response nationallicence Periplasmic protein nationallicence Ars : Arylsulfatase nationallicence CCM : CO2-concentrating mechanism nationallicence dCO2 : Dissolved CO2 nationallicence DIC : Dissolved inorganic carbon nationallicence FeREs : Fe-deficiency-responsive elements nationallicence H43/Fea1 : High-CO2-inducible 43kDa protein/Fe assimilation 1 nationallicence HCR : High-CO2 reporter nationallicence HCRE : High-CO2-responsive cis-element nationallicence PNP : p-Nitrophenol nationallicence PNPS : p-Nitrophenyl sulfate nationallicence Baba Masato Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut Hanawa Yutaka Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut Suzuki Iwane Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut Shiraiwa Yoshihiro Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 169-177 0166-8595 109:1-3<169 2011 109 11120 https://doi.org/10.1007/s11120-010-9619-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-010-9619-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baba Masato Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Hanawa Yutaka Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Suzuki Iwane Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 700 1- Shiraiwa Yoshihiro Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 109/1-3(2011-09-01), 169-177 0166-8595 109:1-3<169 2011 109 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer