caa a22 4500 445889772 CHVBK 20180317145612.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s11120-011-9627-3 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9627-3 Identification of thylakoid membrane thermal transitions in Synechocystis sp. PCC6803 photosynthetic mutants [Elektronische Daten] [Hajnalka Laczkó-Dobos, Svetla Todinova, Özge Sözer, Josef Komenda, Mihály Kis, Anna Sallai, Anelia Dobrikova, Bettina Ughy, Mónika Debreczeny, Zoltán Gombos, Emilia Apostolova, Ildikó Domonkos] We used differential scanning calorimetry (DSC) as a technique capable of identifying photosynthetic complexes on the basis of their calorimetric transitions. Annotation of thermal transitions was carried out with thylakoid membranes isolated from various photosynthetic mutants of Synechocystis sp. PCC6803. The thylakoid membranes exhibited seven major DSC bands between 40 and 85°C. The heat sorption curves were analyzed both by mathematical deconvolution of the overall endotherms and by a subsequent annealing procedure. The successive annealing procedure proved to be more reliable technique than mathematical deconvolution in assigning thermal transitions. The main DSC band, around 47°C, resulting from the high enthalpy change that corresponds to non-interacting complex of PSII, was assigned using the PSI-less/apcE − mutant cells. Another band around 68-70°C relates to the denaturation of PSII surrounded by other proteins of the photosynthetic complexes in wild type and PSI-less/apcE − cells. A further major transition found at 82-84°C corresponds to the PSI core complex of wild type and PSII-deficient BE cells. Other transition bands between 50-67 and 65-75°C are believed to relate to ATP synthase and cytochrome b 6 f, respectively. These thermal transitions were obtained with thylakoids isolated from PSI−/PSII− mutant cells. Some minor bands determined at 59 and 83-84°C correspond to an unknown complex and NADH dehydrogenase, respectively. These annotations were done by PSI-less/apcE − and PSI−/PSII− mutants. Springer Science+Business Media B.V., 2011 Differential scanning calorimetry nationallicence Cyanobacterium nationallicence Photosynthesis nationallicence Thylakoid membrane nationallicence Protein complex nationallicence Endotherm assignation nationallicence 2D-BN/SDS-PAGE : Two-dimensional blue native/SDS polyacrylamide gel electrophoresis nationallicence BE : Phycobilisome-less and PSII-less mutant of Synechocystis PCC6803 nationallicence CC : Core complex nationallicence DSC : Differential scanning calorimetry nationallicence NDH : NADH dehydrogenase nationallicence PSI : Photosystem I nationallicence PSII : Photosystem II nationallicence RC47 : PSII CC without CP43 subunit nationallicence Laczkó-Dobos Hajnalka Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Todinova Svetla Institute of Biophysics, Bulgarian Academy of Sciences, Acad G. Bonchev Bl. 21, 1113, Sofia, Bulgaria aut Sözer Özge Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Komenda Josef Institute of Microbiology, Academy of Sciences, Opatovický mlýn, 37981, Třeboň, Czech Republic aut Kis Mihály Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Sallai Anna Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Dobrikova Anelia Institute of Biophysics, Bulgarian Academy of Sciences, Acad G. Bonchev Bl. 21, 1113, Sofia, Bulgaria aut Ughy Bettina Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Debreczeny Mónika Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Gombos Zoltán Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Apostolova Emilia Institute of Biophysics, Bulgarian Academy of Sciences, Acad G. Bonchev Bl. 21, 1113, Sofia, Bulgaria aut Domonkos Ildikó Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut Photosynthesis Research Springer Netherlands 107/3(2011-03-01), 237-246 0166-8595 107:3<237 2011 107 11120 https://doi.org/10.1007/s11120-011-9627-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9627-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laczkó-Dobos Hajnalka Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Todinova Svetla Institute of Biophysics, Bulgarian Academy of Sciences, Acad G. Bonchev Bl. 21, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Sözer Özge Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Komenda Josef Institute of Microbiology, Academy of Sciences, Opatovický mlýn, 37981, Třeboň, Czech Republic aut NATIONALLICENCE 700 1- Kis Mihály Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Sallai Anna Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Dobrikova Anelia Institute of Biophysics, Bulgarian Academy of Sciences, Acad G. Bonchev Bl. 21, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Ughy Bettina Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Debreczeny Mónika Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Gombos Zoltán Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Apostolova Emilia Institute of Biophysics, Bulgarian Academy of Sciences, Acad G. Bonchev Bl. 21, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Domonkos Ildikó Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 107/3(2011-03-01), 237-246 0166-8595 107:3<237 2011 107 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer