caa a22 4500 445889802 CHVBK 20180317145612.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11120-011-9625-5 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9625-5 A novel membrane based process to isolate photosystem-I membrane complex from spinach [Elektronische Daten] [Jianguo Liu, Mengmeng Yin, Meng Wang, Xuefang Zhang, Baosheng Ge, Shuang Liu, Jianren Lu, Zhanfeng Cui] The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O2 electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone. Springer Science+Business Media B.V., 2011 Ultrafiltration nationallicence Photosystem-I nationallicence Membrane protein nationallicence Spinach nationallicence Activity nationallicence PS-I : Photosystem-I nationallicence DDM : n-Dodecyl-beta-d-maltoside nationallicence PMSF : Phenylmethane-sulfonyl fluoride nationallicence EDTA : Ethylenediaminetetraacetate nationallicence DCIP : 2,6-Dichloroindophenol nationallicence DCMU : 3-(3,4-Dichlorophenyl)-1,1-dimethylurea nationallicence PES : Polyethersulfone nationallicence MWCO : Molecular weight cut-off nationallicence MV : Methylviologen nationallicence PS-II : Photosystem-II nationallicence SDS-PAGE : Sodium dodecyl sulfate-polyacrylamide gel electrophoresis nationallicence CMC : Critical micelle concentration nationallicence TMP : Transmembrane pressure nationallicence Liu Jianguo Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Yin Mengmeng Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Wang Meng Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Zhang Xuefang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Ge Baosheng Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Liu Shuang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut Lu Jianren School of Physics, Manchester University, M60 1QD, Manchester, UK aut Cui Zhanfeng Department of Engineering Science, University of Oxford, OX1 3PJ, Oxford, UK aut Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 187-193 0166-8595 107:2<187 2011 107 11120 https://doi.org/10.1007/s11120-011-9625-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9625-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Jianguo Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Yin Mengmeng Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Wang Meng Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xuefang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Ge Baosheng Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shuang Center for Bioengineering and Biotechnology, China University of Petroleum (East China), 266555, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Lu Jianren School of Physics, Manchester University, M60 1QD, Manchester, UK aut NATIONALLICENCE 700 1- Cui Zhanfeng Department of Engineering Science, University of Oxford, OX1 3PJ, Oxford, UK aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 187-193 0166-8595 107:2<187 2011 107 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer