caa a22 4500 445889810 CHVBK 20180317145612.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11120-010-9609-x doi (NATIONALLICENCE)springer-10.1007/s11120-010-9609-x Effect of cadmium on photosystem II activity in Chlamydomonas reinhardtii [Elektronische Daten] alteration of O-J-I-P fluorescence transients indicating the change of apparent activation energies within photosystem II [François Perreault, Jérôme Dionne, Olivier Didur, Philippe Juneau, Radovan Popovic] In this study, we evaluated how cadmium inhibitory effect on photosystem II and I electron transport may affect light energy conversion into electron transport by photosystem II. To induce cadmium effect on the photosynthetic apparatus, we exposed Chlamydomonas reinhardtii 24h to 0-4.62μM Cd2+. By evaluating the half time of fluorescence transients O-J-I-P at different temperatures (20-30°C), we were able to determine the photosystem II apparent activation energies for different reduction steps of photosystem II, indicated by the O-J-I-P fluorescence transients. The decrease of the apparent activation energies for PSII electron transport was found to be strongly related to the cadmium-induced inhibition of photosynthetic electron transport. We found a strong correlation between the photosystem II apparent activation energies and photosystem II oxygen evolution rate and photosystem I activity. Different levels of cadmium inhibition at photosystem II water-splitting system and photosystem I activity showed that photosystem II apparent activation energies are strongly dependent to photosystem II donor and acceptor sides. Therefore, the oxido-reduction state of whole photosystem II and I electron transport chain affects the conversion of light energy from antenna complex to photosystem II electron transport. Springer Science+Business Media B.V., 2010 Cadmium nationallicence Photosystem II nationallicence Photosystem I nationallicence Oxygen-evolving complex nationallicence Activation energy nationallicence Chlamydomonas reinhardtii nationallicence Perreault François Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut Dionne Jérôme Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut Didur Olivier Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut Juneau Philippe Department of Biological Sciences-TOXEN, Laboratory of Aquatic Microorganism Ecotoxicology, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut Popovic Radovan Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 151-157 0166-8595 107:2<151 2011 107 11120 https://doi.org/10.1007/s11120-010-9609-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-010-9609-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Perreault François Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Dionne Jérôme Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Didur Olivier Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Juneau Philippe Department of Biological Sciences-TOXEN, Laboratory of Aquatic Microorganism Ecotoxicology, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Popovic Radovan Department of Chemistry, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-Ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 151-157 0166-8595 107:2<151 2011 107 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer