caa a22 4500 445889829 CHVBK 20180317145612.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11120-010-9606-0 doi (NATIONALLICENCE)springer-10.1007/s11120-010-9606-0 Use of a SPAD-502 meter to measure leaf chlorophyll concentration in Arabidopsis thaliana [Elektronische Daten] [Qihua Ling, Weihua Huang, Paul Jarvis] The SPAD-502 meter is a hand-held device that is widely used for the rapid, accurate and non-destructive measurement of leaf chlorophyll concentrations. It has been employed extensively in both research and agricultural applications, with a range of different plant species. However, its utility has not been fully exploited in relation to the most intensively studied model organism for plant science research, Arabidopsis thaliana. Measurements with the SPAD-502 meter produce relative SPAD meter values that are proportional to the amount of chlorophyll present in the leaf. In order to convert these values into absolute units of chlorophyll concentration, calibration curves must be derived and utilized. Here, we present calibration equations for Arabidopsis that can be used to convert SPAD values into total chlorophyll per unit leaf area (nmol/cm2; R 2=0.9960) or per unit fresh weight of leaf tissue (nmol/mg; R 2=0.9809). These relationships were derived using a series of Arabidopsis chloroplast biogenesis mutants that exhibit chlorophyll deficiencies of varying severity, and were verified by the subsequent analysis of senescent or light-stressed leaves. Our results revealed that the converted SPAD values differ from photometric measurements of solvent-extracted chlorophyll by just ~6% on average. Springer Science+Business Media B.V., 2010 Arabidopsis nationallicence Calibration curve nationallicence Chlorophyll content nationallicence Chloroplast development nationallicence SPAD meter nationallicence Ling Qihua Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, United Kingdom aut Huang Weihua Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, United Kingdom aut Jarvis Paul Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, United Kingdom aut Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 209-214 0166-8595 107:2<209 2011 107 11120 https://doi.org/10.1007/s11120-010-9606-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-010-9606-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ling Qihua Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, United Kingdom aut NATIONALLICENCE 700 1- Huang Weihua Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, United Kingdom aut NATIONALLICENCE 700 1- Jarvis Paul Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, United Kingdom aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 209-214 0166-8595 107:2<209 2011 107 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer