caa a22 4500 445889888 CHVBK 20180317145612.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s11120-011-9626-4 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9626-4 Excitation energy transfer in the LHC-II trimer: from carotenoids to chlorophylls in space and time [Elektronische Daten] [Jari Martiskainen, Robertas Kananavičius, Juha Linnanto, Heli Lehtivuori, Mika Keränen, Viivi Aumanen, Nikolai Tkachenko, Jouko Korppi-Tommola] Exciton model for description of experimentally determined excitation energy transfer from carotenoids to chlorophylls in the LHC-II trimer of spinach is presented. Such an approach allows connecting the excitonic states to the spatial structure of the complex and hence descriptions of advancements of the initially created excitations in space and time. Carotenoids were excited at 490nm and at 500nm and induced absorbance changes probed in the Chl Qy region to provide kinetic data that were interpreted by using the results from exciton calculations. Calculations included the 42 chlorophylls and the 12 carotenoids of the complex, Soret, Qx and Qy states of the chlorophylls, and the main absorbing S2 state of the carotenoids. According to the calculations excitation at 500nm populates mostly a mixed Lut S2 Chl a Soret state, from where excitation is transferred to the Qx and Qy states of the Chl a's on the stromal side. Internal conversion of the mixed state to a mixed Lut S1 and Chl a Qy state provides a channel for Lut S1 to Chl a Qy energy transfer. The results from the calculations support a picture where excitation at 490nm populates primarily a mixed neoxanthin S2 Chl b Soret state. From this state excitation from neoxanthin is transferred to iso-energetic Chl b Soret states or via internal conversion to S1 Chl b Qy states. From the Soret states excitation proceeds via internal conversion to Qy states of Chl b's mostly on the lumenal side. A rapid Chl b to Chl a transfer and subsequent transfer to the stromal side Chl a's and to the final state completes the process after 490nm excitation. The interpretation is further supported by the fact that excitation energy transfer kinetics after excitation of neoxanthin at 490nm and the Chl b Qy band at 647nm (Linnanto et al., Photosynth Res 87:267-279, 2006) are very similar. Springer Science+Business Media B.V., 2011 Excitation energy transfer nationallicence Light-harvesting complex II nationallicence Carotenoid nationallicence Exciton calculation nationallicence Femtosecond spectroscopy nationallicence Chlorophyll nationallicence Fluorescence up-conversion nationallicence Martiskainen Jari Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut Kananavičius Robertas Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut Linnanto Juha Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut Lehtivuori Heli Tampere University of Technology, P.O. Box 541, 33101, Tampere, Finland aut Keränen Mika Plant Physiology and Molecular Biology, Department of Biochemistry and Food Chemistry, University of Turku, 20014, Turku, Finland aut Aumanen Viivi Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut Tkachenko Nikolai Tampere University of Technology, P.O. Box 541, 33101, Tampere, Finland aut Korppi-Tommola Jouko Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 195-207 0166-8595 107:2<195 2011 107 11120 https://doi.org/10.1007/s11120-011-9626-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9626-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martiskainen Jari Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut NATIONALLICENCE 700 1- Kananavičius Robertas Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut NATIONALLICENCE 700 1- Linnanto Juha Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut NATIONALLICENCE 700 1- Lehtivuori Heli Tampere University of Technology, P.O. Box 541, 33101, Tampere, Finland aut NATIONALLICENCE 700 1- Keränen Mika Plant Physiology and Molecular Biology, Department of Biochemistry and Food Chemistry, University of Turku, 20014, Turku, Finland aut NATIONALLICENCE 700 1- Aumanen Viivi Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut NATIONALLICENCE 700 1- Tkachenko Nikolai Tampere University of Technology, P.O. Box 541, 33101, Tampere, Finland aut NATIONALLICENCE 700 1- Korppi-Tommola Jouko Physical Chemistry Laboratory, Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014, Jyväskylä, Finland aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 107/2(2011-02-01), 195-207 0166-8595 107:2<195 2011 107 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer