caa a22 4500 445889950 CHVBK 20180317145612.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s11120-011-9692-7 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9692-7 Ultrafast time-resolved spectroscopy of the light-harvesting complex 2 (LH2) from the photosynthetic bacterium Thermochromatium tepidum [Elektronische Daten] [Dariusz Niedzwiedzki, Marcel Fuciman, Masayuki Kobayashi, Harry Frank, Robert Blankenship] The light-harvesting complex 2 from the thermophilic purple bacterium Thermochromatium tepidum was purified and studied by steady-state absorption and fluorescence, sub-nanosecond-time-resolved fluorescence and femtosecond time-resolved transient absorption spectroscopy. The measurements were performed at room temperature and at 10K. The combination of both ultrafast and steady-state optical spectroscopy methods at ambient and cryogenic temperatures allowed the detailed study of carotenoid (Car)-to-bacteriochlorophyll (BChl) as well BChl-to-BChl excitation energy transfer in the complex. The studies show that the dominant Cars rhodopin (N=11) and spirilloxanthin (N=13) do not play a significant role as supportive energy donors for BChl a. This is related with their photophysical properties regulated by long π-electron conjugation. On the other hand, such properties favor some of the Cars, particularly spirilloxanthin (N=13) to play the role of the direct quencher of the excited singlet state of BChl. Springer Science+Business Media B.V., 2011 Carotenoids nationallicence Light-harvesting complex 2 nationallicence Transient absorption nationallicence Thermochromatium tepidum nationallicence Rhodopin nationallicence Rhodovibrin nationallicence Spirilloxanthin nationallicence Excited state nationallicence BChl : Bacteriochlorophyll a nationallicence LH2 : Light-harvesting complex 2 nationallicence LH1-RC : Light-harvesting complex 1-reaction center nationallicence ICM : Intracytoplasmic membrane nationallicence DDM : N-Dodecyl β-d-maltoside nationallicence TCSPC : Time-correlated single photon counting nationallicence EET : Efficiency of energy transfer nationallicence RT : Room temperature nationallicence Car : Carotenoid nationallicence VIS : Visible nationallicence NIR : Near-infrared nationallicence ESA : Excited state absorption nationallicence EADS : Evolution-associated difference spectrum nationallicence IRF : Instrument response function nationallicence Niedzwiedzki Dariusz Photosynthetic Antenna Research Center, Washington University in St. Louis, Campus Box 1138, 63130, St. Louis, MO, USA aut Fuciman Marcel Department of Chemistry, University of Connecticut, U-3060, 55 North Eagleville Road, 06269-3060, Storrs, CT, USA aut Kobayashi Masayuki Department of Materials Science and Chemical Engineering, Ariake National College of Technology, 150 Higashihagio-Machi, Omuta, 836-85854, Fukuoka, Japan aut Frank Harry Department of Chemistry, University of Connecticut, U-3060, 55 North Eagleville Road, 06269-3060, Storrs, CT, USA aut Blankenship Robert Photosynthetic Antenna Research Center, Washington University in St. Louis, Campus Box 1138, 63130, St. Louis, MO, USA aut Photosynthesis Research Springer Netherlands 110/1(2011-10-01), 49-60 0166-8595 110:1<49 2011 110 11120 https://doi.org/10.1007/s11120-011-9692-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9692-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Niedzwiedzki Dariusz Photosynthetic Antenna Research Center, Washington University in St. Louis, Campus Box 1138, 63130, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Fuciman Marcel Department of Chemistry, University of Connecticut, U-3060, 55 North Eagleville Road, 06269-3060, Storrs, CT, USA aut NATIONALLICENCE 700 1- Kobayashi Masayuki Department of Materials Science and Chemical Engineering, Ariake National College of Technology, 150 Higashihagio-Machi, Omuta, 836-85854, Fukuoka, Japan aut NATIONALLICENCE 700 1- Frank Harry Department of Chemistry, University of Connecticut, U-3060, 55 North Eagleville Road, 06269-3060, Storrs, CT, USA aut NATIONALLICENCE 700 1- Blankenship Robert Photosynthetic Antenna Research Center, Washington University in St. Louis, Campus Box 1138, 63130, St. Louis, MO, USA aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 110/1(2011-10-01), 49-60 0166-8595 110:1<49 2011 110 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer