caa a22 4500 445889977 CHVBK 20180317145613.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s11120-011-9699-0 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9699-0 Carbonic anhydrase activity in Arabidopsis thaliana thylakoid membrane and fragments enriched with PSI or PSII [Elektronische Daten] [Lyudmila Ignatova, Natalia Rudenko, Vilen Mudrik, Tat'yana Fedorchuk, Boris Ivanov] The procedure of isolating the thylakoids and the thylakoid membrane fragments enriched with either photosystem I or photosystem II (PSI- and PSII-membranes) from Arabidopsis thaliana leaves was developed. It differed from the one used with pea and spinach in durations of detergent treatment and centrifugation, and in concentrations of detergent and Mg2+ in the media. Both the thylakoid and the fragments preserved carbonic anhydrase (CA) activities. Using nondenaturing electrophoresis followed by detection of CA activity in the gel stained with bromo thymol blue, one low molecular mass carrier of CA activity was found in the PSI-membranes, and two carriers, a low molecular mass one and a high molecular mass one, were found in the PSII-membranes. The proteins in the PSII-membranes differed in their sensitivity to acetazolamide (AA), a specific CA inhibitor. AA at 5×10−7M inhibited the CA activity of the high molecular mass protein but stimulated the activity of the low molecular mass carrier in the PSII-membranes. At the same concentration, AA moderately inhibited, by 30%, the CA activity of PSI-membranes. CA activity of the PSII-membranes was almost completely suppressed by the lipophilic CA inhibitor, ethoxyzolamide at 10−9M, whereas CA activity of the PSI-membranes was inhibited by this inhibitor even at 5×10−7M just the same as for AA. The observed distribution of CA activity in the thylakoid membranes from A. thaliana was close to the one found in the membranes of pea, evidencing the general pattern of CA activity in the thylakoid membranes of C3-plants. Springer Science+Business Media B.V., 2011 Carbonic anhydrase nationallicence Arabidopsis thaliana nationallicence Thylakoids nationallicence Photosystem I nationallicence Photosystem II nationallicence Acetazolamide nationallicence Ethoxyzolamide nationallicence AA : Acetazolamide nationallicence CA : Carbonic anhydrase nationallicence Chl : Chlorophyll nationallicence DCBQ : 2,6-Dichloro-p-benzoquinone nationallicence DM : Dodecyl-β-d-maltoside nationallicence EZ : Ethoxyzolamide nationallicence PAAG : Polyacrylamide gel nationallicence PMSF : Phenylmethylsulfonylfluoride nationallicence PSI : Photosystem I nationallicence PSII : Photosystem II nationallicence Rubisco : Ribulosebisphosphatecarboxylase/oxygenase nationallicence TMPD : N,N,N′,N′-tetramethyl-p-phenilene diamine nationallicence Ignatova Lyudmila Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut Rudenko Natalia Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut Mudrik Vilen Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut Fedorchuk Tat'yana Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut Ivanov Boris Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut Photosynthesis Research Springer Netherlands 110/2(2011-12-01), 89-98 0166-8595 110:2<89 2011 110 11120 https://doi.org/10.1007/s11120-011-9699-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9699-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ignatova Lyudmila Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut NATIONALLICENCE 700 1- Rudenko Natalia Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut NATIONALLICENCE 700 1- Mudrik Vilen Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut NATIONALLICENCE 700 1- Fedorchuk Tat'yana Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut NATIONALLICENCE 700 1- Ivanov Boris Institute of Basic Biological Problems of Russian Academy of Sciences, 142290, Pushchino, Moscow, Russia aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 110/2(2011-12-01), 89-98 0166-8595 110:2<89 2011 110 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer