caa a22 4500 445890045 CHVBK 20180317145613.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s11120-011-9647-z doi (NATIONALLICENCE)springer-10.1007/s11120-011-9647-z Structure of PSI, PSII and antennae complexes from yellow-green alga Xanthonema debile [Elektronische Daten] [Zdenko Gardian, Josef Tichý, František Vácha] Photosynthetic carbon fixation by Chromophytes is one of the significant components of a carbon cycle on the Earth. Their photosynthetic apparatus is different in pigment composition from that of green plants and algae. In this work we report structural maps of photosystem I, photosystem II and light harvesting antenna complexes isolated from a soil chromophytic alga Xanthonema debile (class Xanthophyceae). Electron microscopy of negatively stained preparations followed by single particle analysis revealed that the overall structure of Xanthophytes' PSI and PSII complexes is similar to that known from higher plants or algae. Averaged top-view projections of Xanthophytes' light harvesting antenna complexes (XLH) showed two groups of particles. Smaller ones that correspond to a trimeric form of XLH, bigger particles resemble higher oligomeric form of XLH. Springer Science+Business Media B.V., 2011 Photosynthesis nationallicence Chromophytes nationallicence Xanthonema debile nationallicence Photosystem I nationallicence Photosystem II nationallicence FCP nationallicence Electron microscopy nationallicence Chl : Chlorophyll nationallicence HEPES : 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid nationallicence DEAE : Diethyaminoethyl nationallicence DM : n-Dodecyl-β-d-maltoside nationallicence EM : Electron microscopy nationallicence FCP : Fucoxanthin-chlorophyll proteins nationallicence LHCI : Light-harvesting complex of photosystem I nationallicence LHCII : Light-harvesting complex of photosystem II nationallicence PSI : Photosystem I nationallicence PSII : Photosystem II nationallicence SDS-PAGE : Polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate nationallicence Gardian Zdenko Biology Centre ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Tichý Josef Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Vácha František Biology Centre ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Photosynthesis Research Springer Netherlands 108/1(2011-05-01), 25-32 0166-8595 108:1<25 2011 108 11120 https://doi.org/10.1007/s11120-011-9647-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9647-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gardian Zdenko Biology Centre ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Tichý Josef Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Vácha František Biology Centre ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 108/1(2011-05-01), 25-32 0166-8595 108:1<25 2011 108 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer