caa a22 4500 445890185 CHVBK 20180317145613.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9668-7 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9668-7 Identification of C4 photosynthesis metabolism and regulatory-associated genes in Eleocharis vivipara by SSH [Elektronische Daten] [Taiyu Chen, Rongjian Ye, Xiaolei Fan, Xianghua Li, Yongjun Lin] This is the first effort to investigate the candidate genes involved in kranz developmental regulation and C4 metabolic fluxes in Eleocharis vivipara, which is a leafless freshwater amphibious plant and possesses a distinct culms anatomy structure and photosynthetic pattern in contrasting environments. A terrestrial specific SSH library was constructed to investigate the genes involved in kranz anatomy developmental regulation and C4 metabolic fluxes. A total of 73 ESTs and 56 unigenes in 384 clones were identified by array hybridization and sequencing. In total, 50 unigenes had homologous genes in the databases of rice and Arabidopsis. The real-time quantitative PCR results showed that most of the genes were accumulated in terrestrial culms and ABA-induced culms. The C4 marker genes were stably accumulated during the culms development process in terrestrial culms. With respect to C3 culms, C4 photosynthesis metabolism consumed much more transporters and translocators related to ion metabolism, organic acids and carbohydrate metabolism, phosphate metabolism, amino acids metabolism, and lipids metabolism. Additionally, ten regulatory genes including five transcription factors, four receptor-like proteins, and one BURP protein were identified. These regulatory genes, which co-accumulated with the culms developmental stages, may play important roles in culms structure developmental regulation, bundle sheath chloroplast maturation, and environmental response. These results shed new light on the C4 metabolic fluxes, environmental response, and anatomy structure developmental regulation in E. vivipara. Springer Science+Business Media B.V., 2011 Eleocharis vivipara nationallicence C4 photosynthesis metabolic fluxes nationallicence Kranz anatomy development nationallicence Environment and ABA responding nationallicence SSH nationallicence Chen Taiyu National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Ye Rongjian National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Fan Xiaolei National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Li Xianghua National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Lin Yongjun National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 157-170 0166-8595 108:2-3<157 2011 108 11120 https://doi.org/10.1007/s11120-011-9668-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9668-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Taiyu National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Ye Rongjian National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Fan Xiaolei National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Li Xianghua National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Lin Yongjun National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 157-170 0166-8595 108:2-3<157 2011 108 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer