caa a22 4500 445890207 CHVBK 20180317145613.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9662-0 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9662-0 Membrane attachment of Slr0006 in Synechocystis sp. PCC 6803 is determined by divalent ions [Elektronische Daten] [Dalton Carmel, Paula Mulo, Natalia Battchikova, Eva-Mari Aro] Slr0006 is one of the Synechocystis sp. PCC 6803 proteins strongly induced under carbon limiting conditions. Slr0006 has no predicted transmembrane helices or signal peptide sequence, yet it was exclusively recovered in the membrane fraction of Synechocystis, when the cells were broken in isolation buffers which contain divalent cations and are generally used for photosynthesis studies. Even subsequent washing of the membranes with high salt or various detergents did not release Slr0006, indicating strong binding of the Slr0006 protein to the membranes. Further, DNAse or RNAse treatment did not disturb the tight binding of Slr0006 protein to the membranes. Nevertheless, when the cells were broken in the absence of divalent cations, Slr0006 remained completely soluble. Binding of the Slr0006 to the membrane could not be properly reconstituted if the cations were added after breaking the cells in the absence of divalent ions. This unusual phenomenon has to be considered in identification and localization of other yet uncharacterized cyanobacterial proteins. Springer Science+Business Media B.V., 2011 Divalent ions nationallicence Localization nationallicence Membrane nationallicence Slr0006 nationallicence Synechocystis nationallicence Carmel Dalton Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut Mulo Paula Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut Battchikova Natalia Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut Aro Eva-Mari Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 241-245 0166-8595 108:2-3<241 2011 108 11120 https://doi.org/10.1007/s11120-011-9662-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9662-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carmel Dalton Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut NATIONALLICENCE 700 1- Mulo Paula Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut NATIONALLICENCE 700 1- Battchikova Natalia Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut NATIONALLICENCE 700 1- Aro Eva-Mari Laboratory of Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, Tykistökatu 6A, Biocity 6th Floor, 20520, Turku, Finland aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 241-245 0166-8595 108:2-3<241 2011 108 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer