caa a22 4500 445890215 CHVBK 20180317145614.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9678-5 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9678-5 A retrieval algorithm to evaluate the Photosystem I and Photosystem II spectral contributions to leaf chlorophyll fluorescence at physiological temperatures [Elektronische Daten] [Lorenzo Palombi, Giovanna Cecchi, David Lognoli, Valentina Raimondi, Guido Toci, Giovanni Agati] A new computational procedure to resolve the contribution of Photosystem I (PSI) and Photosystem II (PSII) to the leaf chlorophyll fluorescence emission spectra at room temperature has been developed. It is based on the Principal Component Analysis (PCA) of the leaf fluorescence emission spectra measured during the OI photochemical phase of fluorescence induction kinetics. During this phase, we can assume that only two spectral components are present, one of which is constant (PSI) and the other variable in intensity (PSII). Application of the PCA method to the measured fluorescence emission spectra of Ficus benjamina L. evidences that the temporal variation in the spectra can be ascribed to a single spectral component (the first principal component extracted by PCA), which can be considered to be a good approximation of the PSII fluorescence emission spectrum. The PSI fluorescence emission spectrum was deduced by difference between measured spectra and the first principal component. A single-band spectrum for the PSI fluorescence emission, peaked at about 735nm, and a 2-band spectrum with maxima at 685 and 740nm for the PSII were obtained. A linear combination of only these two spectral shapes produced a good fit for any measured emission spectrum of the leaf under investigation and can be used to obtain the fluorescence emission contributions of photosystems under different conditions. With the use of our approach, the dynamics of energy distribution between the two photosystems, such as state transition, can be monitored in vivo, directly at physiological temperatures. Separation of the PSI and PSII emission components can improve the understanding of the fluorescence signal changes induced by environmental factors or stress conditions on plants. Springer Science+Business Media B.V., 2011 Chlorophyll nationallicence Ficus benjamina L nationallicence Fluorescence emission spectra nationallicence Principal component analysis nationallicence Photosystem I nationallicence Photosystem II nationallicence Chl : Chlorophyll nationallicence ChlF : Chlorophyll fluorescence nationallicence F : Fluorescence nationallicence F685 : Red Chl fluorescence emission band nationallicence F735 : Far-red Chl fluorescence emission band nationallicence F685/F735 : Ratio between the red (685nm) and far-red (735nm) ChlF emission bands nationallicence FWHM : Full width at half maximum nationallicence LSF : Least squares fit nationallicence OIP : Nomenclature for Chl a fluorescence induction nationallicence OI : Photochemical phase nationallicence IP : Thermal phase nationallicence PC : Principal components nationallicence PCA : Principal component analysis nationallicence PPFD : Photosynthetic photon flux density nationallicence PSI : Photosystem I nationallicence PSII : Photosystem II nationallicence SNR : Signal to noise ratio nationallicence Palombi Lorenzo Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut Cecchi Giovanna Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut Lognoli David Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut Raimondi Valentina Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut Toci Guido Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut Agati Giovanni Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 225-239 0166-8595 108:2-3<225 2011 108 11120 https://doi.org/10.1007/s11120-011-9678-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9678-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palombi Lorenzo Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut NATIONALLICENCE 700 1- Cecchi Giovanna Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut NATIONALLICENCE 700 1- Lognoli David Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut NATIONALLICENCE 700 1- Raimondi Valentina Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut NATIONALLICENCE 700 1- Toci Guido Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut NATIONALLICENCE 700 1- Agati Giovanni Istituto di Fisica Applicata ‘Nello Carrara', CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Firenze, Italy aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 225-239 0166-8595 108:2-3<225 2011 108 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer