caa a22 4500 445890223 CHVBK 20180317145614.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9682-9 doi (NATIONALLICENCE)springer-10.1007/s11120-011-9682-9 Photosynthetic energy storage efficiency in Chlamydomonas reinhardtii , based on microsecond photoacoustics [Elektronische Daten] [Chengyi Yan, Oscar Schofield, Zvy Dubinsky, David Mauzerall, Paul Falkowski, Maxim Gorbunov] Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the thermal expansion component due to heat dissipation of absorbed energy and the negative volume change due to electrostriction induced by charge separation in each of the photosystems could be readily distinguished. Kinetic analysis revealed that PSI and PSII RCs exhibit strikingly different PA signals where PSI is characterized by a strong electrostriction signal and a weak thermal expansion component while PSII has a small electrostriction component and large thermal expansion. The calculated ES efficiencies at ~10μs were estimated to be 80±5 and 50±13% for PSII-deficient mutants and PSI-deficient mutants, respectively, and 67±2% for WT. The overall ES efficiency was positively correlated with the ratio of PSI to PSI+PSII. Our results suggest that the shallow excitonic trap in PSII limits the efficiency of ES as a result of an evolutionary frozen metabolic framework of two photosystems in all oxygenic photoautotrophs. Springer Science+Business Media B.V., 2011 Energy storage efficiency nationallicence Photoacoustics nationallicence Photosystem I nationallicence Photosystem II nationallicence Chlamydomonas reinhardtii nationallicence Yan Chengyi Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut Schofield Oscar Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut Dubinsky Zvy Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel aut Mauzerall David Rockefeller University, 10065, New York, NY, USA aut Falkowski Paul Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut Gorbunov Maxim Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 215-224 0166-8595 108:2-3<215 2011 108 11120 https://doi.org/10.1007/s11120-011-9682-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9682-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Chengyi Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Schofield Oscar Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Dubinsky Zvy Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel aut NATIONALLICENCE 700 1- Mauzerall David Rockefeller University, 10065, New York, NY, USA aut NATIONALLICENCE 700 1- Falkowski Paul Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Gorbunov Maxim Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, 08901, New Brunswick, NJ, USA aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 215-224 0166-8595 108:2-3<215 2011 108 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer