caa a22 4500 445890258 CHVBK 20180317145614.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s11120-011-9680-y doi (NATIONALLICENCE)springer-10.1007/s11120-011-9680-y Adaptation of photosystem II to high and low light in wild-type and triazine-resistant Canola plants: analysis by a fluorescence induction algorithm [Elektronische Daten] [Jack van Rensen, Wim Vredenberg] Plants of wild-type and triazine-resistant Canola (Brassica napus L.) were exposed to very high light intensities and after 1day placed on a laboratory table at low light to recover, to study the kinetics of variable fluorescence after light, and after dark-adaptation. This cycle was repeated several times. The fast OJIP fluorescence rise curve was measured immediately after light exposure and after recovery during 1day in laboratory room light. A fluorescence induction algorithm has been used for resolution and analysis of these curves. This algorithm includes photochemical and photo-electrochemical quenching release components and a photo-electrical dependent IP-component. The analysis revealed a substantial suppression of the photo-electrochemical component (even complete in the resistant biotype), a partial suppression of the photochemical component and a decrease in the fluorescence parameter F o after high light. These effects were recovered after 1day in the indoor light. The Author(s), 2011 Canola ( Brassica napus L.) nationallicence Chlorophyll fluorescence induction algorithm nationallicence Photoinhibition nationallicence Reaction kinetics nationallicence Triazine-resistance nationallicence β : Fraction (S 0) of QB-nonreducing RCs in dark-adapted system nationallicence CET : Cyclic electron transport in photosystem I nationallicence Chl : Chlorophyll nationallicence dsq : Donor side quenching by the secondary electron donor of photosystem II, Yz + nationallicence F m : Maximal fluorescence when degree of primary photochemical—(q PP) and of photoelectrochemical quenching (q PE) and photoelectroelectric stimulation (q CET)=0, i.e., when q PP=q PE=q CET=0 nationallicence F m STF : Maximal fluorescence after single turnover flash nationallicence F o : Minimal fluorescence when q PP=q PE=q CET=1 (see also definition of F m) nationallicence F v : Variable fluorescence=F−F o nationallicence FIA : Fluorescence induction algorithm nationallicence F PE( t ) : Simulated fluorescence emission at time t, relative to F o, associated with release of photoelectrochemical quenching at full and invariable primary photochemical quenching (q PP=1) major contributor of F v in the O-J -phase nationallicence F PP( t ) : Simulated fluorescence emission at time t, relative to F o, associated with release of primary photochemical quenching at full and invariable photo-electrochemical quenching (q PE=1) major contributor of F v in the J-I- phase nationallicence F CET( t ) : Simulated fluorescence emission at time t, relative to F o, associated with photo-electric stimulation at full and invariable primary photochemical- and photo-electrochemical quenching (q PP=q PE=1) major contributor of F v in the I-P- phase nationallicence k AB : Rate constant of QA − oxidation nationallicence k Hthyl : Rate constant of trans-thylakoid proton leak (conductance) nationallicence k IP : Rate constant of the simulated photoelectrical stimulation (F CET(t)) fitting the major part of the experimental I-P phase nationallicence k L : Rate constant of the excitation by actinic light nationallicence k qbf : Rate constant associated with accumulation and reduction of QB-nonreducing RCs nationallicence nF v : Normalized variable fluorescence (F−F o)/F o nationallicence q dsq : Fraction of PSII RCs in which acceptor- and donor-side quenching by Y+ and QA −, respectively, is released nationallicence q PP : Degree of primary photochemical quenching with 0≤q PP≤1 nationallicence q PE : Degree of photoelectrochemical quenching with 0≤q PE≤1 nationallicence q CET : Degree of photoelectrical F v stimulation with 0≤q CET≤1 nationallicence HL : High light nationallicence LL : Low light nationallicence MTF : Multi-turnover flash (light pulse) nationallicence N IP : Integer (0<N IP<10) to accommodate delay and steepness of (F CET(t)) response in simulating the variable fluorescence during the I-P- phase nationallicence OEC : Oxygen evolving complex nationallicence PSII (I) : Photosystem II (I) nationallicence QA : Primary quinone acceptor of PSII nationallicence QB : Secondary quinone acceptor of PSII nationallicence RC : Reaction center of PS nationallicence STF : Single turnover flash (excitation) nationallicence van Rensen Jack Laboratory of Plant Physiology, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Vredenberg Wim Laboratory of Plant Physiology, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 191-200 0166-8595 108:2-3<191 2011 108 11120 https://doi.org/10.1007/s11120-011-9680-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11120-011-9680-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Rensen Jack Laboratory of Plant Physiology, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Vredenberg Wim Laboratory of Plant Physiology, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Photosynthesis Research Springer Netherlands 108/2-3(2011-09-01), 191-200 0166-8595 108:2-3<191 2011 108 11120 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer