caa a22 4500 445890940 CHVBK 20180317145616.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10750-011-0650-3 doi (NATIONALLICENCE)springer-10.1007/s10750-011-0650-3 A novel, combined approach to assessing species delimitation and biogeography within the well-known desmid species Micrasterias fimbriata and M. rotata (Desmidiales, Steptophyta) [Elektronische Daten] [Jiří Neustupa, Jan Št'astný, Katarína Nemjová, Petra Mazalová, Emma Goodyer, Aloisie Poulíčková, Pavel Škaloud] Morphological species of freshwater microalgae often have broad geographic distribution. However, traditional species concepts have been challenged by the results of molecular phylogenetic analyses that mostly indicate higher diversity than was previously recognized by purely morphological approaches. A degree of phenotypic differentiation or different geographic distribution of species defined by molecular data remains largely unknown. In this study, we analyzed a pair of well-known and widely distributed desmid species (Micrasterias fimbriata and M. rotata) and tested for their phylogenetic and morphological homogeneity as well as their geographic distribution. Geometric morphometric and morphological attributes of cells were used in combination with genetic analysis of the trnG ucc sequences of 30 strains isolated from a variety of European locations and obtained from culture collections. Micrasterias rotata proved to be phylogenetically homogenous across Europe while M. fimbriata turned out to be composed of two firmly delimited lineages, differing by molecular as well as by morphometric and morphological data. Published records of traditional M. fimbriata were also included in the classification discrimination analysis and were placed into the newly identified lineages upon comparison to the morphometric data collected from living material. Largely disparate geographic patterns were revealed within traditional M. fimbriata. One phylogenetic lineage is frequent in central and eastern Europe, but occurs also in the British Isles. A second lineage has been recorded in North America and in Western Europe, where its distribution is possibly limited to the west of the Rhine River. Interestingly, the morphometric analyses of the published records illustrated that the geographic differences have remained largely unchanged since the 1850s indicating a previously unknown distributional stability among microalgal species groups such as the desmids. Springer Science+Business Media B.V., 2011 Microalgae distribution nationallicence Desmidiales nationallicence Geometric morphometrics nationallicence Green algae nationallicence Molecular phylogenetics nationallicence Micrasterias nationallicence trnG ucc nationallicence Neustupa Jiří Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut Št'astný Jan Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut Nemjová Katarína Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut Mazalová Petra Department of Botany, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46, Olomouc, Czech Republic aut Goodyer Emma Royal Botanic Garden Edinburgh (RBGE), 20a Inverleith Row, EH3 5LR, Edinburgh, UK aut Poulíčková Aloisie Department of Botany, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46, Olomouc, Czech Republic aut Škaloud Pavel Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut Hydrobiologia Springer Netherlands 667/1(2011-06-01), 223-239 0018-8158 667:1<223 2011 667 10750 https://doi.org/10.1007/s10750-011-0650-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-011-0650-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neustupa Jiří Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut NATIONALLICENCE 700 1- Št'astný Jan Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut NATIONALLICENCE 700 1- Nemjová Katarína Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut NATIONALLICENCE 700 1- Mazalová Petra Department of Botany, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Goodyer Emma Royal Botanic Garden Edinburgh (RBGE), 20a Inverleith Row, EH3 5LR, Edinburgh, UK aut NATIONALLICENCE 700 1- Poulíčková Aloisie Department of Botany, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Škaloud Pavel Department of Botany, Faculty of Science, Charles University of Prague, Benátská 2, 128 01, Praha, Czech Republic aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 667/1(2011-06-01), 223-239 0018-8158 667:1<223 2011 667 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer