caa a22 4500 445891033 CHVBK 20180317145616.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10750-011-0763-8 doi (NATIONALLICENCE)springer-10.1007/s10750-011-0763-8 Chl- a fluorescence parameters as biomarkers of metal toxicity in fluvial biofilms: an experimental study [Elektronische Daten] [Natàlia Corcoll, Berta Bonet, Manel Leira, Helena Guasch] A study was carried out to evaluate the sensitivity of different chlorophyll-a (chl-a) fluorescence parameters measured in freshwater biofilms as metal pollution biomarkers of short- and long-term metal exposures at environmentally realistic concentrations. A microcosm experiment was performed using indoor channels. Mature biofilms were exposed from hours to weeks to three different treatments: No-Metal, Zn (400μgl−1); and Zn plus Cd (400μgl−1 and 20μg l−1, respectively). Metal concentration was based on a real case study: the Riou-Mort River (France). Biofilms exposed to Zn bioaccumulated similar Zn contents per dry weight to those exposed to the mixture (Zn plus Cd) causing a similar inhibition of the effective quantum yield $$(\Upphi_{\text{M}}^{\prime})$$ during the first hours of exposure. A reduction of the algal biomass, a shift in the community composition (a high reduction of diatoms), a reduction of the maximal quantum yield (ΦM) and a strong reduction of non-photochemical quenching (NPQ) were observed from day 14 until the end of the experiment (35days). The results indicate that the effects of the metal mixture present in the Riou-Mort on biofilms could be attributed to Zn toxicity. The use of a set of chl-a fluorescence measurements, including photochemical and NPQ parameters, are recommended as a reliable biomarker tool box to evaluate both short- and long-term effects of metals on biofilms containing oxygenic photoautotrophs, suggesting its use in field applications. Springer Science+Business Media B.V., 2011 Fluvial biofilm nationallicence Metals nationallicence Pulse-amplitude-modulated (PAM) fluorometry nationallicence Bioaccumulation nationallicence Indoor channels nationallicence Corcoll Natàlia Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071, Girona, Spain aut Bonet Berta Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071, Girona, Spain aut Leira Manel Faculty of Sciences, University of A Coruña, Campus da Zapateira, 15071, A Coruña, Spain aut Guasch Helena Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071, Girona, Spain aut Hydrobiologia Springer Netherlands 673/1(2011-09-01), 119-136 0018-8158 673:1<119 2011 673 10750 https://doi.org/10.1007/s10750-011-0763-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-011-0763-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Corcoll Natàlia Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071, Girona, Spain aut NATIONALLICENCE 700 1- Bonet Berta Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071, Girona, Spain aut NATIONALLICENCE 700 1- Leira Manel Faculty of Sciences, University of A Coruña, Campus da Zapateira, 15071, A Coruña, Spain aut NATIONALLICENCE 700 1- Guasch Helena Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071, Girona, Spain aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 673/1(2011-09-01), 119-136 0018-8158 673:1<119 2011 673 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer