caa a22 4500 445891181 CHVBK 20180317145616.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10750-011-0749-6 doi (NATIONALLICENCE)springer-10.1007/s10750-011-0749-6 Genetic variation within and between populations of hermaphroditic Bulinus truncatus tetraploid freshwater snails of the Albertine Rift, East Africa [Elektronische Daten] [Allen Nalugwa, Aslak Jørgensen, Silvester Nyakaana, Thomas Kristensen] Genetic variability within and among Bulinus truncatus of the Albertine Rift freshwater bodies were assessed to investigate the degree of inbreeding and gene flow in the snail populations. The effect of ploidy on the genetic structuring of B.truncatus is also described. We characterized the genetic structure of seven B.truncatus populations from Lake Albert, Lake Kivu, and Katosho swamp in Tanzania using five polymorphic microsatellite loci. Genetic differentiation was quantified using pairwise FST values and Nei's standard genetic distances. Different alleles were observed across all loci and genetic diversity was low although it varied greatly across populations; observed heterozygosity was, however, higher than the expected heterozygosity in three of the populations studied. Significant heterozygote deficiencies were observed coupled with significant linkage disequilibria in five populations for all the five loci examined in this study. We found significant genetic differentiation among the seven freshwater bodies; private alleles were observed across all loci indicating restricted or absence of gene flow between populations. Limited snail dispersal and the reproductive biology of B.truncatus are the major forces shaping the genetic variation observed. Low genetic variation within B.truncatus populations exposes them to a high parasite infection risk as predicted in the Red Queen hypothesis. Springer Science+Business Media B.V., 2011 Bulinus truncatus nationallicence Microsatellites nationallicence Genetic structure nationallicence Albertine Rift nationallicence Nalugwa Allen Molecular Biology Laboratory, Makerere University, College of Agricultural and Environmental Sciences, P.O. Box 7298, Kampala, Uganda aut Jørgensen Aslak Laboratory of Molecular Systematics, The Natural History Museum of Denmark, Sølvgade 83, 1307, Copenhagen K, Denmark aut Nyakaana Silvester Molecular Biology Laboratory, Makerere University, College of Agricultural and Environmental Sciences, P.O. Box 7298, Kampala, Uganda aut Kristensen Thomas The Mandahl-Barth Research Centre for Biodiversity and Health, DBL-Parasitology, Health and Development, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 57, 1871, Frederiksberg C, Denmark aut Hydrobiologia Springer Netherlands 673/1(2011-09-01), 53-61 0018-8158 673:1<53 2011 673 10750 https://doi.org/10.1007/s10750-011-0749-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-011-0749-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nalugwa Allen Molecular Biology Laboratory, Makerere University, College of Agricultural and Environmental Sciences, P.O. Box 7298, Kampala, Uganda aut NATIONALLICENCE 700 1- Jørgensen Aslak Laboratory of Molecular Systematics, The Natural History Museum of Denmark, Sølvgade 83, 1307, Copenhagen K, Denmark aut NATIONALLICENCE 700 1- Nyakaana Silvester Molecular Biology Laboratory, Makerere University, College of Agricultural and Environmental Sciences, P.O. Box 7298, Kampala, Uganda aut NATIONALLICENCE 700 1- Kristensen Thomas The Mandahl-Barth Research Centre for Biodiversity and Health, DBL-Parasitology, Health and Development, Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 57, 1871, Frederiksberg C, Denmark aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 673/1(2011-09-01), 53-61 0018-8158 673:1<53 2011 673 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer