caa a22 4500 445891432 CHVBK 20180317145617.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10750-010-0453-y doi (NATIONALLICENCE)springer-10.1007/s10750-010-0453-y Regional structure despite limited mtDNA sequence diversity found in the endangered Huchen, Hucho hucho (Linnaeus, 1758) [Elektronische Daten] [S. Weiss, S. Marić, A. Snoj] We evaluate the hypothesis of no geographic structure in the Huchen (Hucho hucho), a large, predatory salmonid endemic to the Danube basin. Forty-seven individuals sampled from throughout the Huchen's native range were genetically characterized. Extremely limited sequence diversity across 1,800 bases of mtDNA (the complete control region and partial NADH-1 subunit) evidenced by four closely related mtDNA haplotypes was found. Nonetheless, the geographic distribution of mtDNA repeats (5-10, 82-bp long copies per individual) as well as allelic diversity across two microsatellite loci indicated large-scale geographic structure between the north-western (Austria and Slovenia) distribution area and eastern (Slovakia and Ukraine) or southern (Bosnia-Herzegovina and Montenegro) sample sites. An extremely slow rate of substitution for the H. hucho mtDNA is considered along with the alternative hypotheses to explain the limited mtDNA diversity. Considering the regional genetic structure implied by our data, we advocate restrictions on the transport of brood fish or yearlings across the range of the species distribution and sale of Huchen across international boundaries. Future genetic analysis to support local conservation and monitoring efforts must focus on developing a high-resolution screen that may be applied to identify hatchery versus naturally reproduced individuals in the wild. Springer Science+Business Media B.V., 2010 Danube salmon nationallicence Danubian basin nationallicence Phylogeography nationallicence Microsatellites nationallicence mtDNA nationallicence Weiss S. Institut für Zoologie, Karl-Franzens Universität Graz, Universitätsplatz 2, 8010, Graz, Austria aut Marić S. Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11001, Belgrade, Serbia aut Snoj A. Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230, Domžale, Slovenia aut Hydrobiologia Springer Netherlands 658/1(2011-01-01), 103-110 0018-8158 658:1<103 2011 658 10750 https://doi.org/10.1007/s10750-010-0453-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0453-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weiss S. Institut für Zoologie, Karl-Franzens Universität Graz, Universitätsplatz 2, 8010, Graz, Austria aut NATIONALLICENCE 700 1- Marić S. Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11001, Belgrade, Serbia aut NATIONALLICENCE 700 1- Snoj A. Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230, Domžale, Slovenia aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 658/1(2011-01-01), 103-110 0018-8158 658:1<103 2011 658 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer