caa a22 4500 445892137 CHVBK 20180317145619.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10750-011-0724-2 doi (NATIONALLICENCE)springer-10.1007/s10750-011-0724-2 Presence and genetic diversity of microcystin-producing cyanobacteria ( Anabaena and Microcystis ) in Lake Kotokel (Russia, Lake Baikal Region) [Elektronische Daten] [Olga Belykh, Ekaterina Sorokovikova, Galina Fedorova, Oksana Kaluzhnaya, Evgeniya Korneva, Maria Sakirko, Tatyana Sherbakova] A survey was conducted for the presence of cyanobacteria toxins in Lake Kotokel due to a few cases of Haff disease registered in 2008-2009 caused by consumption of fish from Lake Kotokel, and wildlife mortality including large fish kill. The aims of this study were to determine what cyanotoxins (if any) were present in the lake, to describe phytoplankton composition including morphology, density, and species diversity of cyanobacteria, as well as to evaluate the trophic state of the lake. Samples were collected from both nearshore and central sites in August of 2009. Aphanocapsa holsatica dominated the phytoplankton. The presence of toxigenic genotypes of Microcystis spp. and Anabaena lemmermannii was detected by sequencing of PCR-amplified aminotransferase domain of microcystin synthetase gene. LR, RR, and YR microcystin (MC) variants were detected with liquid chromatography-UV mass spectrometry. The data do not shed light on the etiology of Haff disease in Lake Kotokel region, nevertheless taking into account the recreational importance of the lake and its direct connection to Lake Baikal, a necessity to monitor cyanobacteria in these water bodies is evident. This is the first report on simultaneous detection of MC-producing genotypes and MCs in the Lake Baikal region. Springer Science+Business Media B.V., 2011 Toxic cyanobacteria nationallicence Microcystin nationallicence mcy E gene nationallicence HPLC nationallicence LC/MS nationallicence Lake Kotokel nationallicence Lake Baikal nationallicence Belykh Olga Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Sorokovikova Ekaterina Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Fedorova Galina Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Kaluzhnaya Oksana Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Korneva Evgeniya Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Sakirko Maria Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Sherbakova Tatyana Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut Hydrobiologia Springer Netherlands 671/1(2011-08-01), 241-252 0018-8158 671:1<241 2011 671 10750 https://doi.org/10.1007/s10750-011-0724-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-011-0724-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Belykh Olga Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Sorokovikova Ekaterina Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Fedorova Galina Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Kaluzhnaya Oksana Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Korneva Evgeniya Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Sakirko Maria Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 700 1- Sherbakova Tatyana Limnological Institute of the Siberian Branch of RAS, 3, Ulan-Batorskaya St, 664033, Irkutsk, Russia aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 671/1(2011-08-01), 241-252 0018-8158 671:1<241 2011 671 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer