caa a22 4500 44589251X CHVBK 20180317145621.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10750-010-0478-2 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0478-2 Isolation of mixis-related genes from the rotifer Brachionus plicatilis using subtractive hybridization [Elektronische Daten] [Koushirou Suga, Natsuki Oshiyama, Yukari Tanaka, Yoshitaka Sakakura, Atsushi Hagiwara] The monogonont rotifer Brachionus plicatilis produces resting eggs through sexual reproduction (mixis), which is affected by external and internal factors. We collected resting eggs from rotifers cultured at 15 and 25°C and hatched them with and without 14-day dormancy in the dark. Stem females hatched from both conditions were further cultured at 15, 20 and 25°C. We obtained two phenotypes, one with high mixis (more than 50%), which was hatched from resting eggs formed at 15°C without dormancy, and another in which sexual reproduction occurred at 25°C and resting eggs were formed at 15°C with a 14-day dormancy. In the latter phenotype, mictic females appeared at 15°C, but not at 25°C. Using subtractive hybridization, we isolated one gene from the latter phenotype of females that had no significant similarity to known genes in BLAST searches. We propose that this gene is unique to rotifer mictic reproduction. Ongoing characterization of this gene attempts understand its role in mixis. Springer Science+Business Media B.V., 2010 Rotifera nationallicence Brachionus plicatilis nationallicence Mixis-related gene nationallicence Temperature regulation nationallicence Suga Koushirou Faculty of Fisheries, Nagasaki University, Nagasaki, Japan aut Oshiyama Natsuki Graduate School of Science and Technology, Nagasaki University, Nagasaki, Japan aut Tanaka Yukari Faculty of Fisheries, Nagasaki University, Nagasaki, Japan aut Sakakura Yoshitaka Faculty of Fisheries, Nagasaki University, Nagasaki, Japan aut Hagiwara Atsushi Graduate School of Science and Technology, Nagasaki University, Nagasaki, Japan aut Hydrobiologia Springer Netherlands 662/1(2011-03-01), 83-88 0018-8158 662:1<83 2011 662 10750 https://doi.org/10.1007/s10750-010-0478-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0478-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suga Koushirou Faculty of Fisheries, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 700 1- Oshiyama Natsuki Graduate School of Science and Technology, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 700 1- Tanaka Yukari Faculty of Fisheries, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 700 1- Sakakura Yoshitaka Faculty of Fisheries, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hagiwara Atsushi Graduate School of Science and Technology, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 662/1(2011-03-01), 83-88 0018-8158 662:1<83 2011 662 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer