caa a22 4500 445892609 CHVBK 20180317145621.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10750-010-0519-x doi (NATIONALLICENCE)springer-10.1007/s10750-010-0519-x Effect of experimental methodology on estimation of density at sex initiation in cyclically parthenogenetic rotifers [Elektronische Daten] [M. Carmona, N. Dimas-Flores, J. Montero-Pau, M. Serra] Abstracts: Sexual reproduction is density-dependent in several cyclically parthenogenetic rotifer species and induced by a chemical signal produced by the rotifers. As population density increases, the chemical accumulates in the medium until reaching a threshold that triggers sex. The density threshold for sex initiation is of ecological and evolutionary importance due to the large impact that timing of sex has on fitness in cyclical parthenogenetic rotifers. Here we study the effects of experimental methodology in combination with intrinsic features of the biological system on the estimation of the density required to produce enough of this chemical to initiate sex (i.e., density threshold). We have developed a theoretical model describing the dynamics of both population density and the concentration of the chemical signal. Model analysis showed that the time at which sex is recorded in an experiment, the initial density (i.e., experimental volume) and the population growth rate can affect the estimation of density thresholds. Results from an experiment in which density at sex initiation was tested for 29 clones of Brachionus plicatilis at two culture volumes, revealed that density threshold is negatively dependent on the experimental volume. We conclude that in order to estimate density thresholds so that they are comparable across studies, experimental methodology should be standardized. Springer Science+Business Media B.V., 2010 Density at sex initiation nationallicence Population density threshold nationallicence Mixis inducing protein nationallicence Theoretical model nationallicence Volume effect nationallicence Methodological approach nationallicence Carmona M. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut Dimas-Flores N. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut Montero-Pau J. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut Serra M. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut Hydrobiologia Springer Netherlands 662/1(2011-03-01), 131-139 0018-8158 662:1<131 2011 662 10750 https://doi.org/10.1007/s10750-010-0519-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0519-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carmona M. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut NATIONALLICENCE 700 1- Dimas-Flores N. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut NATIONALLICENCE 700 1- Montero-Pau J. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut NATIONALLICENCE 700 1- Serra M. Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Apartado Oficial 22085, 46071, Valencia, Spain aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 662/1(2011-03-01), 131-139 0018-8158 662:1<131 2011 662 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer