caa a22 4500 445892668 CHVBK 20180317145621.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10750-010-0516-0 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0516-0 Sequence analysis of genomic DNA (680 Mb) by GS-FLX-Titanium sequencer in the monogonont rotifer, Brachionus ibericus [Elektronische Daten] [Jae-Seong Lee, Ryeo-Ok Kim, Jae-Sung Rhee, Jeonghoon Han, Dae-Sik Hwang, Beom-Soon Choi, Chang Lee, Yong-Dal Yoon, Jong-Sung Lim, Young-Mi Lee, Gyung Park, Atsushi Hagiwara, Ik-Young Choi] The monogonont rotifer, Brachionus ibericus (S type), is considered to be a promising model species for developmental biology, evolution, and environmental genomics. In an attempt to accelerate the molecular understanding of B. ibericus, we sequenced 680.5Mb of genomic DNA using the genome sequencer GS-FLX-Titanium. We obtained 2,062,621 reads (average read length 329.9bp) and 145,418 contigs (total contigs length 125.7Mb) after excluding small reads (less than 200bp) from the assembly, and finally obtained 10,133 unigenes (E value≤9.00E−04) after non-redundant (NR) BLAST search. In this article, we summarize the genomic DNA sequences of B. ibericus and discuss their potential use in the study of reproductive biology, endocrinology, environmental genomics, and ecotoxicological studies, and for providing insight into the genetic basis of mechanisms such as egg formation, antioxidant stress defense, and xenobiotic metabolism. Springer Science+Business Media B.V., 2010 Mongonont rotifer nationallicence Brachionus ibericus nationallicence GS-FLX-Titanium nationallicence Genomic DNA nationallicence Unigene nationallicence Lee Jae-Seong National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut Kim Ryeo-Ok National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut Rhee Jae-Sung Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, 133-791, Seoul, South Korea aut Han Jeonghoon Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, 133-791, Seoul, South Korea aut Hwang Dae-Sik Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, 133-791, Seoul, South Korea aut Choi Beom-Soon National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut Lee Chang Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut Yoon Yong-Dal Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut Lim Jong-Sung National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, 151-742, Seoul, South Korea aut Lee Young-Mi Department of Green Life Science, College of Convergence, Sangmyung University, 110-743, Seoul, South Korea aut Park Gyung Department of Marine Biotechnology, College of Liberal Arts and Sciences, Anyang University, 417-833, Ganghwa, South Korea aut Hagiwara Atsushi Graduate School of Science and Technology, Nagasaki University, 852-8521, Nagasaki, Japan aut Choi Ik-Young National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, 151-742, Seoul, South Korea aut Hydrobiologia Springer Netherlands 662/1(2011-03-01), 65-75 0018-8158 662:1<65 2011 662 10750 https://doi.org/10.1007/s10750-010-0516-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0516-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Jae-Seong National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Ryeo-Ok National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Rhee Jae-Sung Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Han Jeonghoon Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Hwang Dae-Sik Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Choi Beom-Soon National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, and Research Institute for Natural Sciences, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Chang Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Yoon Yong-Dal Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, South Korea aut NATIONALLICENCE 700 1- Lim Jong-Sung National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, 151-742, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Young-Mi Department of Green Life Science, College of Convergence, Sangmyung University, 110-743, Seoul, South Korea aut NATIONALLICENCE 700 1- Park Gyung Department of Marine Biotechnology, College of Liberal Arts and Sciences, Anyang University, 417-833, Ganghwa, South Korea aut NATIONALLICENCE 700 1- Hagiwara Atsushi Graduate School of Science and Technology, Nagasaki University, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 700 1- Choi Ik-Young National Instrumentation Center for Environmental Management, College of Agriculture and Life Sciences, Seoul National University, 151-742, Seoul, South Korea aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 662/1(2011-03-01), 65-75 0018-8158 662:1<65 2011 662 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer