caa a22 4500 445892757 CHVBK 20180317145621.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10750-010-0485-3 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0485-3 Development of genomic resources for the phylogenetic analysis of the Brachionus plicatilis species complex (Rotifera: Monogononta) [Elektronische Daten] [Javier Montero-Pau, Africa Gómez] There has been a substantial leap forward in the quantity and quality of genomic resources available for research on rotifers in recent years. We used available genomic and bioinformatics resources to identify variable regions of the genome to design PCR and sequencing primers for the Brachionus plicatilis complex. We then tested their suitability for the study of systematics of this group. Eight amplified successfully for members of the B.plicatilis complex. We sequenced the amplified products, constructed a concatenated alignment (5,511bp) and carried out phylogenetic analyses. The resulting tree, based on mitochondrial and nuclear genes for 11 clones of six species, was well supported even at relatively deep nodes, contrasting with results of previous studies using only mitochondrial genes, which provide little phylogenetic information for the deepest nodes. The same procedure could be used to design primers for more conserved regions to be used in a wider range of rotifer groups. Springer Science+Business Media B.V., 2010 EST library nationallicence Bioinformatics nationallicence Phylogenetics nationallicence Iberian Peninsula nationallicence Primer development nationallicence mtDNA nationallicence Montero-Pau Javier Department of Biological Sciences, University of Hull, HU6 7RX, Hull, UK aut Gómez Africa Department of Biological Sciences, University of Hull, HU6 7RX, Hull, UK aut Hydrobiologia Springer Netherlands 662/1(2011-03-01), 43-50 0018-8158 662:1<43 2011 662 10750 https://doi.org/10.1007/s10750-010-0485-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0485-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montero-Pau Javier Department of Biological Sciences, University of Hull, HU6 7RX, Hull, UK aut NATIONALLICENCE 700 1- Gómez Africa Department of Biological Sciences, University of Hull, HU6 7RX, Hull, UK aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 662/1(2011-03-01), 43-50 0018-8158 662:1<43 2011 662 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer