caa a22 4500 445892803 CHVBK 20180317145622.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10750-010-0572-5 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0572-5 Morphological, biochemical and molecular characterization of Anabaena , Aphanizomenon and Nostoc strains (Cyanobacteria, Nostocales) isolated from Portuguese freshwater habitats [Elektronische Daten] [Victor Galhano, Daniela de Figueiredo, Artur Alves, António Correia, Mário Pereira, José Gomes-Laranjo, Francisco Peixoto] Studies of cyanobacterial nostocacean taxa are important to the global scientific community, mainly because a significant number of beneficial strains that belong to the order Nostocales fix atmospheric nitrogen, thus contributing to the fertility of agricultural soils worldwide, while others behave as nuisance microorganisms in aquatic ecosystems due to their involvement in toxic bloom events. However, in spite of their ecological importance and environmental concerns, their identification and taxonomy are still problematic and doubtful, often being based on current morphological and physiological studies, which generate confusing classification systems and usually vary under different conditions. Therefore, the present research aimed to investigate through a polyphasic approach differences in morphological, biochemical and genotypic features of three nostocacean cyanobacterial strains isolated from central-western Portuguese shallow freshwater bodies. Morphometric, genetic (16S rRNA, nifH and hetR fragments) and biochemical (fatty acid methyl ester; FAME profiles) data were used to characterize the strains. Morphological analysis and sequencing of 16S rRNA fragments showed that the strains belonged to Anabaena cylindrica (UTAD_A212), Aphanizomenon gracile (UADFA16) and Nostoc muscorum (UTAD_N213) species. These strains showed clear distinct morphological and genetic features, allowing easy allocation to their respective genera. The same happened by using partial sequences of hetR and nifH genes, in spite of the scarcity of deposited sequences. Biochemical characterization showed that the FAME profiles obtained were consistent with both morphological and molecular analyses. It was suggested that the ratio of monounsaturated to polyunsaturated FAMEs, together with the unsaturation index, could be used as genus-specific chemotaxonomic biomarkers. Springer Science+Business Media B.V., 2010 Polyphasic taxonomy nationallicence Cyanobacteria nationallicence 16S rRNA nationallicence hetR nationallicence nifH nationallicence Fatty acid methyl esters nationallicence Galhano Victor Department of Biology and Environment, CITAB—Centre for Research and Technology of Agro-Environment and Biological Sciences, Sustainable Agro-Food Chains Research Group, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal aut de Figueiredo Daniela Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut Alves Artur Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut Correia António Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut Pereira Mário Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut Gomes-Laranjo José Department of Biology and Environment, CITAB—Centre for Research and Technology of Agro-Environment and Biological Sciences, Sustainable Agro-Food Chains Research Group, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal aut Peixoto Francisco Department of Chemistry, CECAV—Centre of Animal Sciences and Veterinary, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal aut Hydrobiologia Springer Netherlands 663/1(2011-03-01), 187-203 0018-8158 663:1<187 2011 663 10750 https://doi.org/10.1007/s10750-010-0572-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0572-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Galhano Victor Department of Biology and Environment, CITAB—Centre for Research and Technology of Agro-Environment and Biological Sciences, Sustainable Agro-Food Chains Research Group, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal aut NATIONALLICENCE 700 1- de Figueiredo Daniela Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Alves Artur Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Correia António Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Pereira Mário Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Gomes-Laranjo José Department of Biology and Environment, CITAB—Centre for Research and Technology of Agro-Environment and Biological Sciences, Sustainable Agro-Food Chains Research Group, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal aut NATIONALLICENCE 700 1- Peixoto Francisco Department of Chemistry, CECAV—Centre of Animal Sciences and Veterinary, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 663/1(2011-03-01), 187-203 0018-8158 663:1<187 2011 663 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer