caa a22 4500 445892854 CHVBK 20180317145622.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10750-010-0574-3 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0574-3 Seasonal and spatial variability of sediment bacterial communities inhabiting the large shallow Lake Balaton [Elektronische Daten] [Gitta Szabó, Bernadett Khayer, Anna Rusznyák, István Tátrai, György Dévai, Károly Márialigeti, Andrea Borsodi] Seasonal studies of surface sediment bacterial communities, from two basins with differing trophic states within Lake Balaton (Hungary), were carried out using molecular (denaturing gradient gel electrophoresis, DGGE) and cultivation-based techniques. The presence of polyphosphate accumulates was tested using Neisser staining, and phosphatase activity was investigated on organic phosphorus (P) compound. Aerobic viable cell counts were significantly higher in the eutrophic than mesotrophic basin in each season. The lowest viable counts were observed in the autumn and the highest in spring and summer month in both basins. The DGGE fingerprints of the samples reflected that the composition of sediment bacterial communities in the two basins were distinct in spring and summer, and similar in autumn, but similarly diverse in all seasons. On the basis of partial 16S rDNA sequences, the 216 strains were affiliated with six major bacterial lineages: Firmicutes; Actinobacteria, Bacteroidetes, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. Common species characterized from both basins constituted up to 66% of all identified phylotypes. Strains related to Bacillus sp. were dominant in all but one sample. Isolates affiliated with Aeromonas sp. prevailed in the sample taken from the mesotrophic basin in spring. The majority of the strains showed excess poly-P accumulation. Association of Neisser staining and phosphatase activity test results suggested that excess poly-P accumulation serves as P storage for sediment bacteria. Our study implied the importance of Firmicutes, Actinobacteria, Alphaproteobacteria, and Aeromonas species in benthic bacterial P retention. Springer Science+Business Media B.V., 2010 Lake Balaton nationallicence Sediment bacterial communities nationallicence DGGE nationallicence Cultivation nationallicence Polyphosphate-accumulating bacteria nationallicence Szabó Gitta Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut Khayer Bernadett Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut Rusznyák Anna Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut Tátrai István Balaton Limnological Research Institute of the Hungarian Academy of Sciences, 3 Klebelsberg Kuno út, 8237, Tihany, Hungary aut Dévai György Department of Hydrobiology, University of Debrecen, 1 Egyetem tér, 4032, Debrecen, Hungary aut Márialigeti Károly Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut Borsodi Andrea Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut Hydrobiologia Springer Netherlands 663/1(2011-03-01), 217-232 0018-8158 663:1<217 2011 663 10750 https://doi.org/10.1007/s10750-010-0574-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0574-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Szabó Gitta Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Khayer Bernadett Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Rusznyák Anna Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Tátrai István Balaton Limnological Research Institute of the Hungarian Academy of Sciences, 3 Klebelsberg Kuno út, 8237, Tihany, Hungary aut NATIONALLICENCE 700 1- Dévai György Department of Hydrobiology, University of Debrecen, 1 Egyetem tér, 4032, Debrecen, Hungary aut NATIONALLICENCE 700 1- Márialigeti Károly Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Borsodi Andrea Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, 1117, Budapest, Hungary aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 663/1(2011-03-01), 217-232 0018-8158 663:1<217 2011 663 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer