caa a22 4500 44589329X CHVBK 20180317145623.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10750-011-0815-0 doi (NATIONALLICENCE)springer-10.1007/s10750-011-0815-0 Phenotype and temperature affect the affinity for dissolved inorganic carbon in a cyanobacterium Microcystis [Elektronische Daten] [Xinghua Wu, Zhongxing Wu, Lirong Song] The cyanobacterium Microcystis is the most common bloom-forming species in eutrophicated water bodies. Known eco-physiological advantages of this organism help it to compete effectively with other algae and cyanobacteria; however, little is known about the physiological characteristics competence of colonial Microcystis. In the present study, carbonic anhydrase (CA) activity, the affinity for dissolved inorganic carbon (DIC), and the transcription of CA genes were examined in unicellular and colonial Microcystis strains. In comparison with unicellular strains, colonial Microcystis exhibited dramatically higher inorganic carbon affinity at 25 and 35°C, but no significant differences were observed at 15°C. The relative transcription levels of the CA genes icfA1, icfA2, ecaA, and ecaB in all colonial Microcystis were significantly higher than those in unicellular Microcystis at 25 and 35°C. In addition, CA activities of Microcystis increased with temperature, but no significant difference was observed between the unicellular and colonial Microcystis. These results suggest that temperature and phenotypes probably play important roles in the utilization of DIC and trigger the expression of CA genes of Microcystis. The present results may indicate that the capacity for utilizing inorganic carbon plays a role in the persistence or/and succession of different Microcystis blooms. Springer Science+Business Media B.V., 2011 Phenotype nationallicence Colonial Microcystis nationallicence Dissolved inorganic carbon nationallicence Carbonic anhydrase nationallicence Wu Xinghua State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, People's Republic of China aut Wu Zhongxing State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, People's Republic of China aut Song Lirong State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, People's Republic of China aut Hydrobiologia Springer Netherlands 675/1(2011-10-01), 175-186 0018-8158 675:1<175 2011 675 10750 https://doi.org/10.1007/s10750-011-0815-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-011-0815-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Xinghua State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Wu Zhongxing State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 700 1- Song Lirong State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, People's Republic of China aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 675/1(2011-10-01), 175-186 0018-8158 675:1<175 2011 675 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer