caa a22 4500 445893567 CHVBK 20180317145624.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10750-010-0510-6 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0510-6 Molecular and microscopic diversity of planktonic eukaryotes in the oligotrophic Lake Stechlin (Germany) [Elektronische Daten] [W. Luo, C. Bock, H. Li, J. Padisák, L. Krienitz] The aim of this study was to compare a molecular and a microscopic approach to study the planktonic eukaryotic diversity of an oligotrophic lake. Plankton samples from the temperate Lake Stechlin were assessed in winter and summer 2008 by comparison of 18S rRNA gene clone libraries to light microscopic evaluations. For both approaches identical samples were used. There were remarkable differences between the main groups recovered by the contrasting methods. The microscopic analyses showed predominance of autotrophic planktonic organisms, whereas most of them could not be discovered by the molecular method which resulted in a higher diversity of heterotrophic flagellates. The microscopic survey revealed high diversity of Chlorophyta and Cryptophyta as well as the Stramenopiles groups of Bacillariophyceae and Chrysophyceae. The clone libraries, based on full-length 18S rRNA gene sequences, displayed highest diversity of Alveolata belonging to seven different subclades. Notably, Antarctic Dinophyta-related clones were detected. The occurrence of the marine phagotrophic flagellate Telonema was also documented. Comparing the two sampling seasons, rich diversity suggests that flagellates played an important role in late winter (February), however, there is relatively low diversity in summer (August). The newly discovered molecular diversity of planktonic eukaryotes in Stechlin will help to understand the biodiversity patterns in freshwater lakes. Springer Science+Business Media B.V., 2010 Microbial eukaryotes nationallicence Microscopy nationallicence Molecular biodiversity nationallicence Lake Stechlin nationallicence Luo W. SOA Key Laboratory for Polar Science, Polar Research Institute of China, 200136, Shanghai, China aut Bock C. Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Stechlin-Neuglobsow, Germany aut Li H. SOA Key Laboratory for Polar Science, Polar Research Institute of China, 200136, Shanghai, China aut Padisák J. Department of Limnology, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary aut Krienitz L. Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Stechlin-Neuglobsow, Germany aut Hydrobiologia Springer Netherlands 661/1(2011-02-01), 133-143 0018-8158 661:1<133 2011 661 10750 https://doi.org/10.1007/s10750-010-0510-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0510-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo W. SOA Key Laboratory for Polar Science, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 700 1- Bock C. Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Stechlin-Neuglobsow, Germany aut NATIONALLICENCE 700 1- Li H. SOA Key Laboratory for Polar Science, Polar Research Institute of China, 200136, Shanghai, China aut NATIONALLICENCE 700 1- Padisák J. Department of Limnology, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary aut NATIONALLICENCE 700 1- Krienitz L. Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Stechlin-Neuglobsow, Germany aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 661/1(2011-02-01), 133-143 0018-8158 661:1<133 2011 661 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer