caa a22 4500 445894385 CHVBK 20180317145627.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10750-010-0586-z doi (NATIONALLICENCE)springer-10.1007/s10750-010-0586-z Effects of animal density, volume, and the use of 2D/3D recording on behavioral studies of copepods [Elektronische Daten] [Gaël Dur, Sami Souissi, François Schmitt, François-Gaël Michalec, Mohamed-Sofiane Mahjoub, Jiang-Shiou Hwang] Studies on the behavior of copepods require both an appropriate experimental design and the means to perform objectively verifiable numerical analysis. Despite the growing number of publications on copepod behavior, it has been difficult to compare these studies. In this study, we studied two species of copepods, Eurytemora affinis and Pseudodiaptomus annandalei, and employed recently developed scaling and non-scaling methodology to investigate the effects of density and volume on the swimming behavior of individual organisms in still water. We also compared the results of two- and three-dimensional projections of the swimming tracks. A combination of scale-dependent and scale-independent analysis was found to characterize a number of behavioral observations very effectively. We discovered that (i) density has no effect except to increase the time spent in the swimming state of "breaking”, (ii) smaller volumes resulted in more complex trajectories, and larger volumes, like density, increased the time spent in the swimming state "breaking”, and (iii) three-dimensional projections gave a more accurate estimation of speed and the time spent cruising. When only a vertical 2D projection was used, "cruising” could be confused with "sinking”. These results indicate that both experimental conditions and the selection of 2D or 3D projection have important implications regarding the study of copepod behavior. The development of standardized procedures with which to compare the observations made in different studies is an issue of particular urgency. Springer Science+Business Media B.V., 2010 Swimming behavior nationallicence Analysis nationallicence Methodology nationallicence Calanoid copepod nationallicence Eurytemora affinis nationallicence Pseudodiaptomus annandalei nationallicence Dur Gaël Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut Souissi Sami Université Lille Nord de France, 59000, Lille, France aut Schmitt François Université Lille Nord de France, 59000, Lille, France aut Michalec François-Gaël Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut Mahjoub Mohamed-Sofiane Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut Hwang Jiang-Shiou Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut Hydrobiologia Springer Netherlands 666/1(2011-05-01), 197-214 0018-8158 666:1<197 2011 666 10750 https://doi.org/10.1007/s10750-010-0586-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0586-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dur Gaël Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut NATIONALLICENCE 700 1- Souissi Sami Université Lille Nord de France, 59000, Lille, France aut NATIONALLICENCE 700 1- Schmitt François Université Lille Nord de France, 59000, Lille, France aut NATIONALLICENCE 700 1- Michalec François-Gaël Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut NATIONALLICENCE 700 1- Mahjoub Mohamed-Sofiane Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut NATIONALLICENCE 700 1- Hwang Jiang-Shiou Institute of Marine Biology, National Taiwan Ocean University, 2 Pei-ning Road, 20224, Keelung, Taiwan, ROC aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 666/1(2011-05-01), 197-214 0018-8158 666:1<197 2011 666 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer