caa a22 4500 445894563 CHVBK 20180317145627.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10750-010-0590-3 doi (NATIONALLICENCE)springer-10.1007/s10750-010-0590-3 Onset of and recovery from physiological stress in Liocarcinus depurator after trawling and air exposure under different seasonal conditions [Elektronische Daten] [S. Raicevich, F. Giomi, F. Pranovi, O. Giovanardi, P. Di Muro, M. Beltramini] The onset of and recovery from physiological stress in Liocarcinus depurator (Decapoda: Portunidae), that were trawled and subjected to emersion during fishing activities, was analysed in a field study in the Northern Adriatic Sea. Our working hypothesis intended to assess the development of physiological impairment due to air exposure during sorting operations, and the possible recovery trajectories after the return to sea of this by-caught species. The protocol we used included experimental trawling and quantification of physiological stress indicators (haemolymph concentrations of l-lactate, d-glucose, ammonia and pH) under different seasonal conditions. Immediately after being caught, when the fishing net was emptied on board, L.depurator showed higher physiological imbalance in the summer vs. winter experiments, highlighting the immediate effect of temperature shock due to the difference in temperature between the sea bottom and the deck (15-18 and 2°C in summer and winter, respectively). Experimental animals in the permanently emersed condition exhibited a progressive disruption of homeostasis in both seasons, as confirmed by a significant increase in lactate and a decrease in the pH of the haemolymph as a function of the emersion time. Ammonia levels were almost stable in the summer, when the physiological limits of this metabolite were reached immediately after animals were caught. In the winter, a significant increase in ammonia was observed over the time. This pattern can be attributed to the impairment of gill function, resulting in suffocation (manifested by marked haemolymph acidosis) and reduced ammonia excretion. Glucose concentration was revealed to be stable during air exposure in both seasons, though a higher concentration was recorded in the summer compared to the winter. The recovered individuals tended to return to pre-caught values for all of the haemolymph parameters we measured, though with different trajectories in the two seasons. From these results, we conclude that temperature shock, coupled with air exposure, influenced the ultimate stress level of this species to a greater extent than other effects of fishing. Springer Science+Business Media B.V., 2011 Physiological stress nationallicence Emersion nationallicence Temperature shock nationallicence Recovery nationallicence Fishing disturbance nationallicence Decapods nationallicence Raicevich S. ISPRA, Istituto Superiore per la Protezione e la Ricerca Ambientale, Loc. Brondolo, 30015, Chioggia (Venezia), Italy aut Giomi F. Department of Biology, University of Padova, Viale G. Colombo 3, 35131, Padova, Italy aut Pranovi F. Enviromental Sciences, Informatics and Statistic Department, Università Ca' Foscari, Castello 2737/b, 30122, Venice, Italy aut Giovanardi O. ISPRA, Istituto Superiore per la Protezione e la Ricerca Ambientale, Loc. Brondolo, 30015, Chioggia (Venezia), Italy aut Di Muro P. Department of Biology, University of Padova, Viale G. Colombo 3, 35131, Padova, Italy aut Beltramini M. Department of Biology, University of Padova, Viale G. Colombo 3, 35131, Padova, Italy aut Hydrobiologia Springer Netherlands 664/1(2011-04-01), 107-118 0018-8158 664:1<107 2011 664 10750 https://doi.org/10.1007/s10750-010-0590-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10750-010-0590-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raicevich S. ISPRA, Istituto Superiore per la Protezione e la Ricerca Ambientale, Loc. Brondolo, 30015, Chioggia (Venezia), Italy aut NATIONALLICENCE 700 1- Giomi F. Department of Biology, University of Padova, Viale G. Colombo 3, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Pranovi F. Enviromental Sciences, Informatics and Statistic Department, Università Ca' Foscari, Castello 2737/b, 30122, Venice, Italy aut NATIONALLICENCE 700 1- Giovanardi O. ISPRA, Istituto Superiore per la Protezione e la Ricerca Ambientale, Loc. Brondolo, 30015, Chioggia (Venezia), Italy aut NATIONALLICENCE 700 1- Di Muro P. Department of Biology, University of Padova, Viale G. Colombo 3, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Beltramini M. Department of Biology, University of Padova, Viale G. Colombo 3, 35131, Padova, Italy aut NATIONALLICENCE 773 0- Hydrobiologia Springer Netherlands 664/1(2011-04-01), 107-118 0018-8158 664:1<107 2011 664 10750 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer