caa a22 4500 463156110 CHVBK 20180406164734.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00217-006-0499-2 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0499-2 Analysis of element species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS [Elektronische Daten] [Oto Mestek, Jan Polák, Richard Koplík, Jana Komínková, Jiří Šantrůček, Milan Kodíček, František Kvasnička] Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn in extracts of buckwheat and amaranth flour in 0.02mol·l−1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS. All metals except for Fe and Mn (both samples) and Cu (amaranth only) are concentrated in a low-molecular fraction (M r<2kDa). Fe is bound in a high-molecular fraction (M r>150 kDa). The main part of Mn is present as a labile complex or an ionic form. Cu in amaranth extract is also bound in medium-molecular fractions (10 and 40kDa) and in a high-molecular one (M r>150kDa). Fractions rich in trace metals were isolated using preparative-scale SEC and the organic ligands of metals were refined by immobilized metal affinity chromatography. Low content of sulphur-containing amino acids (Cys, Met) and high content of acidic amino acids (Glu, Asp) are typical for all these purified fractions. A part of Zn (buckwheat) and almost all Cu (amaranth) in the low-molecular fractions were located in phosphorus rich sub-fractions. However, all Ni (both samples), the majority of Zn (buckwheat) and all Cu (buckwheat) were found in phosphorus-less sub-fractions. No phytochelatins were found in MALDI-MS spectra of isolated ligands. Springer-Verlag, 2006 Amaranth nationallicence Buckwheat nationallicence Speciation nationallicence Trace elements nationallicence MALDI-MS nationallicence ICP-MS nationallicence Mestek Oto Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Polák Jan Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Koplík Richard Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Komínková Jana Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Šantrůček Jiří Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Kodíček Milan Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Kvasnička František Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 895-904 1438-2377 225:5-6<895 2007 225 217 https://doi.org/10.1007/s00217-006-0499-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0499-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mestek Oto Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Polák Jan Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Koplík Richard Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Komínková Jana Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Šantrůček Jiří Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Kodíček Milan Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Kvasnička František Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 895-904 1438-2377 225:5-6<895 2007 225 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer