caa a22 4500 463156250 CHVBK 20180406164735.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00217-006-0477-8 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0477-8 Immumochromatographic assay for determination of hexoestrol residues [Elektronische Daten] [Tieming Huo, Chifang Peng, Chuanlai Xu, Liqiang Liu] One-step membrane-based competitive colloidal gold-based immunoassays in lateral-flow formats for the rapid detection of hexoestrol (HES) were developed. Nitrocellulose membrane strip was separately coated with goat anti-rabbit IgG (control line) and HES hapten-OVA conjugate (test line). Anti-HES polyclonal antibody labelled with colloidal gold particles was firstly incubated with HES. A positive reaction as a result of the remaining antibody-gold conjugate combining with antigen coated on the membrane was obvious by visual detection, with detection limits for lateral flow of 10μg/kg for detecting HES standard solution, and the limit of detection was 20μg/kg for detecting the HES spiked in swine urine and liver. The assay time for test was less than 5min, suitable for rapid testing on-site. Springer-Verlag, 2006 HES nationallicence Lateral-flow nationallicence Colloidal gold-based immunoassay nationallicence Huo Tieming School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut Peng Chifang School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut Xu Chuanlai School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut Liu Liqiang School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 743-747 1438-2377 225:5-6<743 2007 225 217 https://doi.org/10.1007/s00217-006-0477-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0477-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huo Tieming School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut NATIONALLICENCE 700 1- Peng Chifang School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut NATIONALLICENCE 700 1- Xu Chuanlai School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut NATIONALLICENCE 700 1- Liu Liqiang School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 743-747 1438-2377 225:5-6<743 2007 225 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer