caa a22 4500 463156277 CHVBK 20180406164735.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00217-006-0498-3 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0498-3 Analysis of phenolic compounds and antioxidant activity with H4IIE cells of three different rice grain varieties [Elektronische Daten] [Hee-Youn Chi, Chang-Ho Lee, Kwang-Ho Kim, Sun-Lim Kim, Ill-Min Chung] The phenolic content and the antioxidant capacity of ethanolic extract of rice grains were examined using three different rice (Oryza sativa L. var. japonica) varieties, Jakwangchalbyeo (red pericarp glutinous rice), Hwasunchalbyeo (white pericarp glutinous rice), and Ilpumbyeo (white pericarp non-glutinous rice). Quantitative high performance liquid chromatography (HPLC) analyses showed that Jakwangchalbyeo contains the highest amount of total phenolic substances. Radical scavenging activities measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay were 87.5% (Jakwangchalbyeo), 45.0% (Hwasunchalbyeo), and 50.0% (Ilpumbyeo). The results suggest a positive correlation between the phenolic content and the antioxidation capacity. Antioxidant activity in the ethanolic extract of rice grain was further assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H2O2). Hwasunchalbyeo and Ilpumbyeo extracts showed no significant effects on H2O2-induced oxidative stress. Jakwangchalbyeo extract increased cell viability by up to 82% and 74% after 5- and 24-h treatments at 100μg/mL, respectively. Consistent with the results of DPPH and MTT assays, dichlorofluorescein (DCF) fluorescence was further reduced when H2O2 was applied with Jakwangchalbyeo extract. In conclusion, red pericarp Jakwangchalbyeo grain extracts exerted significantly higher inhibitory effects on H2O2-induced oxidative stress in H4IIE cells than did other white pericarp rice grain extracts. Springer-Verlag, 2006 Oryza sativa L nationallicence Phenolic compounds nationallicence Antioxidant activity nationallicence MTT nationallicence FACS nationallicence DCF fluorescence nationallicence DCF : Dichlorofluorescein nationallicence DCFH-DA : 2′,7′-Dichlorofluorescein diacetate nationallicence DMSO : Dimethylsulfoxide nationallicence DPPH : 1,1-Diphenyl-2-picryl hydrazyl nationallicence FACS : Fluorescence-activated cell sorting nationallicence MTT : 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide nationallicence ROS : Reactive oxygen species nationallicence Chi Hee-Youn Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701, Seoul, South Korea aut Lee Chang-Ho Department of Pharmacology, College of Medicine, Hanyang University, 143-704, Seoul, South Korea aut Kim Kwang-Ho Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701, Seoul, South Korea aut Kim Sun-Lim National Institute of Crop Science, Rural Development Administration (RDA), 441-859, Suwon, South Korea aut Chung Ill-Min Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701, Seoul, South Korea aut European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 887-893 1438-2377 225:5-6<887 2007 225 217 https://doi.org/10.1007/s00217-006-0498-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0498-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chi Hee-Youn Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Chang-Ho Department of Pharmacology, College of Medicine, Hanyang University, 143-704, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Kwang-Ho Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Sun-Lim National Institute of Crop Science, Rural Development Administration (RDA), 441-859, Suwon, South Korea aut NATIONALLICENCE 700 1- Chung Ill-Min Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 887-893 1438-2377 225:5-6<887 2007 225 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer