caa a22 4500 463156307 CHVBK 20180406164735.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s00217-006-0457-z doi (NATIONALLICENCE)springer-10.1007/s00217-006-0457-z Determination of flumequine, nalidixic acid and oxolinic acid in shrimps by high-performance liquid chromatography with fluorescence detection [Elektronische Daten] [Ute Schröder, Anke Machetzki] A simple high-performance liquid chromatography (HPLC) method with fluorescence detection is presented for the determination of the quinolones flumequine, nalidixic acid and oxolinic acid in shrimps. The pharmaceuticals were extracted from the matrix with acetonitrile, purified by liquid-liquid extraction and analysed by HPLC with fluorescence detection at excitation wavelength of 312nm and emission wavelength of 368nm. The method has been validated according to the recommendations of the European Commission Decision 2002/657/EC under the consideration of specificity, trueness and precision as well as decision limit CCα and detection capability CCβ. The results of the validation demonstrate that the method is suitable to verify surely quinolone residues in shrimps and to decide with an estimated certainty if the investigated sample complies the European Council Regulation 2377/90/EEC or not. Furthermore, shrimp retail products of the years 2004 and 2005 were investigated with the developed HPLC-method. Springer-Verlag, 2006 Quinolones nationallicence Antibiotics nationallicence Validation nationallicence Food analysis nationallicence Shrimps nationallicence Schröder Ute Department of Fish Quality, Federal Research Centre of Nutrition and Food, Palmaille 9, 22767, Hamburg, Germany aut Machetzki Anke Department of Fish Quality, Federal Research Centre of Nutrition and Food, Palmaille 9, 22767, Hamburg, Germany aut European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 627-635 1438-2377 225:5-6<627 2007 225 217 https://doi.org/10.1007/s00217-006-0457-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0457-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schröder Ute Department of Fish Quality, Federal Research Centre of Nutrition and Food, Palmaille 9, 22767, Hamburg, Germany aut NATIONALLICENCE 700 1- Machetzki Anke Department of Fish Quality, Federal Research Centre of Nutrition and Food, Palmaille 9, 22767, Hamburg, Germany aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 225/5-6(2007-09-01), 627-635 1438-2377 225:5-6<627 2007 225 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer