caa a22 4500 463156714 CHVBK 20180406164736.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00217-006-0529-0 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0529-0 Evidence of mixed wild populations of Oenococcus oeni strains during wine spontaneous malolactic fermentations [Elektronische Daten] [Isabel López, Carmen Tenorio, Myriam Zarazaga, Marta Dizy, Carmen Torres, Fernanda Ruiz-Larrea] Two hundred and four bacterial isolates from Rioja red wines undergoing spontaneous malolactic fermentation (MLF) were studied. Bacterial species was determined both by microbiological identification methods and by specific PCR analysis. Oenococcus oeni was shown to be the predominant species (98.5% of total isolates). Pulsed field gel electrophoresis (PFGE) of chromosomal DNA digested with SfiI was used to differentiate individual strains of O. oeni. A wide variety of restriction digest patterns were detected, which indicated a rich biodiversity of indigenous strains. Most fermentations (37 out of 41) showed from 2 to 6 clones growing in the same tank. Five O. oeni strains were the most frequently found, appearing in more than three of the 13 studied wineries, and most times in combination with other less frequently found strains. PFGE was shown to be a suitable method for strain differentiation, for monitoring individual strains and determining which strains actually survive and carry out MLF. A high genotypic heterogeneity of wild O. oeni strains was demonstrated and 90% of the studied wines showed mixed populations of O. oeni strains during MLF. Springer-Verlag, 2006 Oenococcus oeni nationallicence Wine nationallicence PFGE nationallicence Spontaneous malolactic fermentation nationallicence López Isabel Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut Tenorio Carmen Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut Zarazaga Myriam Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut Dizy Marta Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut Torres Carmen Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut Ruiz-Larrea Fernanda Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 215-223 1438-2377 226:1-2<215 2007 226 217 https://doi.org/10.1007/s00217-006-0529-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0529-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- López Isabel Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut NATIONALLICENCE 700 1- Tenorio Carmen Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut NATIONALLICENCE 700 1- Zarazaga Myriam Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut NATIONALLICENCE 700 1- Dizy Marta Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut NATIONALLICENCE 700 1- Torres Carmen Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut NATIONALLICENCE 700 1- Ruiz-Larrea Fernanda Department of Food and Agriculture, University of La Rioja, Av. Madre de Dios 51, 26006, Logroño, Spain aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 215-223 1438-2377 226:1-2<215 2007 226 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer