caa a22 4500 463156730 CHVBK 20180406164736.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00217-006-0510-y doi (NATIONALLICENCE)springer-10.1007/s00217-006-0510-y Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H2O2-mediated DNA damage [Elektronische Daten] [Gin-Nae Ahn, Kil-Nam Kim, Seon-Heui Cha, Choon-Bok Song, Jehee Lee, Moon-Soo Heo, In-Kyu Yeo, Nam-Ho Lee, Young-Heun Jee, Jin-Soo Kim, Min-Soo Heu, You-Jin Jeon] The potential antioxidant activities of three phlorotannins (phloroglucinol, eckol and dieckol) purified from Ecklonia cava collected in Jeju Island were investigated to evaluate their potential value as the natural products for foods or cosmetic application. In this study, antioxidant activities were measured by electron spin resonance spectrometry (ESR) technique for scavenging effects of free radicals such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), alkyl, hydroxyl (HO•) and superoxide anion radical (O2 •−) and by comet assay for protecting effects against H2O2-mediated DNA damage. The results show that all the phlorotannins have the potential DPPH, alkyl, hydroxyl and superoxide radical scavenging activities. Especially, eckol samples scavenged around 93% of DPPH at 0.25, 0.5, 1mg/mL of concentrations and were higher than the other phlorotannins, such as phloroglucinol and dieckol samples. Also, protecting effects of the phlorotannins against H2O2-mediated DNA damage increased with increased concentrations of the samples in the L5178 mouse T-cell lymphoma cell lines (L5178Y-R). In conclusion, these results suggest that the three phlorotannins purified from E. cava have the potential inhibitory effect on H2O2-mediated DNA damage and harmful free radicals and can be used as antioxidants in cosmetic, foods and drug industry. Springer-Verlag, 2006 Antioxidant activity nationallicence Ecklonia cava nationallicence Phlorotannin nationallicence Electron spin resonance (ESR) nationallicence Comet assay nationallicence DNA damage nationallicence Ahn Gin-Nae Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Kim Kil-Nam Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Cha Seon-Heui Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Song Choon-Bok Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Lee Jehee Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Heo Moon-Soo Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Yeo In-Kyu Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut Lee Nam-Ho Department of Chemistry, Cheju National University, 690-756, Jeju, Korea aut Jee Young-Heun Department of Veterinary Medicine, Applied Radiological Science Institute, Cheju National University, 690-756, Jeju, Korea aut Kim Jin-Soo Division of Marine Bioscience, Gyeongsang National University, 143-130, Seoul, Korea aut Heu Min-Soo Division of Marine Bioscience, Gyeongsang National University, 143-130, Seoul, Korea aut Jeon You-Jin Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 71-79 1438-2377 226:1-2<71 2007 226 217 https://doi.org/10.1007/s00217-006-0510-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0510-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahn Gin-Nae Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Kim Kil-Nam Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Cha Seon-Heui Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Song Choon-Bok Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Lee Jehee Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Heo Moon-Soo Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Yeo In-Kyu Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Lee Nam-Ho Department of Chemistry, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Jee Young-Heun Department of Veterinary Medicine, Applied Radiological Science Institute, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 700 1- Kim Jin-Soo Division of Marine Bioscience, Gyeongsang National University, 143-130, Seoul, Korea aut NATIONALLICENCE 700 1- Heu Min-Soo Division of Marine Bioscience, Gyeongsang National University, 143-130, Seoul, Korea aut NATIONALLICENCE 700 1- Jeon You-Jin Faculty of Applied Marine Science, Cheju National University, 690-756, Jeju, Korea aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 71-79 1438-2377 226:1-2<71 2007 226 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer