caa a22 4500 463156781 CHVBK 20180406164736.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00217-006-0533-4 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0533-4 Influence of cocoa components on the PCR detection of soy lecithin DNA [Elektronische Daten] [Nicolas Gryson, Koen Dewettinck, Kathy Messens] The labelling of genetically modified organisms (GMOs) is mandatory in the EU. To comply with this regulation, the International Standardisation Organisation recently published standards for the extraction of DNA and PCR detection of GMOs. The PCR detection of (GM) soy lecithin in chocolate however is not an easy assignment. For the most part this is due to inhibitors such as polyphenols resulting from the cocoa mass and the low level of soy lecithin present. Many factors however, such as the choice of extraction method, sample size and PCR protocol influence the PCR end result. Therefore, preconditions regarding the quality, quantity and purity of the extracted DNA should be carefully determined to guarantee a successful GMO analysis. Springer-Verlag, 2006 Chocolate nationallicence GMO detection nationallicence Lecithin nationallicence PCR inhibition nationallicence ISO nationallicence Gryson Nicolas Laboratory AgriFing, Faculty of Biosciences and Landscape Architecture, University College Ghent, Ghent University Association, Voskenslaan 270, 9000, Ghent, Belgium aut Dewettinck Koen Laboratory of Food Technology and Engineering, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, 9000, Ghent, Belgium aut Messens Kathy Laboratory AgriFing, Faculty of Biosciences and Landscape Architecture, University College Ghent, Ghent University Association, Voskenslaan 270, 9000, Ghent, Belgium aut European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 247-254 1438-2377 226:1-2<247 2007 226 217 https://doi.org/10.1007/s00217-006-0533-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0533-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gryson Nicolas Laboratory AgriFing, Faculty of Biosciences and Landscape Architecture, University College Ghent, Ghent University Association, Voskenslaan 270, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Dewettinck Koen Laboratory of Food Technology and Engineering, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Messens Kathy Laboratory AgriFing, Faculty of Biosciences and Landscape Architecture, University College Ghent, Ghent University Association, Voskenslaan 270, 9000, Ghent, Belgium aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 247-254 1438-2377 226:1-2<247 2007 226 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer