caa a22 4500 463156862 CHVBK 20180406164736.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00217-006-0536-1 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0536-1 Physical degradation of genomic DNA of soybean flours does not impair relative quantification of its transgenic content [Elektronische Daten] [Frédéric Debode, Eric Janssen, Gilbert Berben] In this paper, four different physical treatments (microwaves, heating by conduction, sonication and pressure autoclaving) were performed to degrade a pure DNA extract, and their influence on GMO quantification was studied. The aim was to check the hypothesis that processing of agrofood products results in a similar degradation rate for both the transgenic target and the specific target. Indeed we could observe that even if the used physical treatments could lead to a significant increase of C t values for both transgenic and specific targets, the resulting ΔC t remained stable. So, the main conclusion of the study is that the aforementioned hypothesis seems valid and thus a physical degradation of DNA will not affect the relative quantitation of the GMO content, provided that both the specific and transgenic targets have very similar size. A second important issue of the experiments performed was that DNA is a very robust molecule as it is extremely difficult to reach a mean size below 100bp. The study also gives evidence of the importance of using small targets. Springer-Verlag, 2006 GMO nationallicence Quantification nationallicence Real-time PCR nationallicence Roundup Ready soybean nationallicence Food analysis nationallicence Debode Frédéric Department Quality of Agricultural Products, Walloon Agricultural Research Center (CRA-W), 24, chaussée de Namur, 5030, Gembloux, Belgium aut Janssen Eric Department Quality of Agricultural Products, Walloon Agricultural Research Center (CRA-W), 24, chaussée de Namur, 5030, Gembloux, Belgium aut Berben Gilbert Department Quality of Agricultural Products, Walloon Agricultural Research Center (CRA-W), 24, chaussée de Namur, 5030, Gembloux, Belgium aut European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 273-280 1438-2377 226:1-2<273 2007 226 217 https://doi.org/10.1007/s00217-006-0536-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0536-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Debode Frédéric Department Quality of Agricultural Products, Walloon Agricultural Research Center (CRA-W), 24, chaussée de Namur, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Janssen Eric Department Quality of Agricultural Products, Walloon Agricultural Research Center (CRA-W), 24, chaussée de Namur, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Berben Gilbert Department Quality of Agricultural Products, Walloon Agricultural Research Center (CRA-W), 24, chaussée de Namur, 5030, Gembloux, Belgium aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 273-280 1438-2377 226:1-2<273 2007 226 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer