caa a22 4500 463156978 CHVBK 20180406164737.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s00217-006-0502-y doi (NATIONALLICENCE)springer-10.1007/s00217-006-0502-y A procedural approach for the identification of sources of uncertainty associated with GM quantification and real-time quantitative PCR measurements [Elektronische Daten] [Malcolm Burns, Hernan Valdivia] Estimation of measurement uncertainty is important as it provides objective evidence that an assay or instrument is ‘fit for purpose', and is a requirement for accreditation to ISO17025. Technological developments within the bioanalytical laboratory have progressed rapidly in the past few years. However, the measurement science associated with these technologies has not yet fully matured. The provision of a full measurement uncertainty budget is often difficult because of a lack of standardised approaches and the constraints associated with time, finances and expertise. A procedural approach for the identification of factors that can account for measurement uncertainty in quantification of genetically modified (GM) ingredients using real-time quantitative PCR is presented here. This example illustrates an approach for the identification of factors that can be applied to related assays concerned with GM quantification, can be utilised as part of existing uncertainty approaches, and can provide the basis for making quantitative estimates of uncertainty that will contribute towards a full measurement uncertainty budget. Springer-Verlag, 2006 Measurement uncertainty nationallicence GMOs nationallicence Real-time quantitative PCR nationallicence Burns Malcolm Bio-Molecular Innovation, LGC Limited, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut Valdivia Hernan Bio-Molecular Innovation, LGC Limited, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 7-18 1438-2377 226:1-2<7 2007 226 217 https://doi.org/10.1007/s00217-006-0502-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0502-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burns Malcolm Bio-Molecular Innovation, LGC Limited, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut NATIONALLICENCE 700 1- Valdivia Hernan Bio-Molecular Innovation, LGC Limited, Queens Road, TW11 0LY, Teddington, Middlesex, UK aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 226/1-2(2007-11-01), 7-18 1438-2377 226:1-2<7 2007 226 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer