caa a22 4500 463157052 CHVBK 20180406164737.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00217-006-0368-z doi (NATIONALLICENCE)springer-10.1007/s00217-006-0368-z Effects of β-carotene on soybean lipoxygenase activity: kinetic studies [Elektronische Daten] [Arda Serpen, Vural Gökmen] β-carotene directly influences the amount of soybean lipoxygenase (LOX) in the reaction medium available for the catalytic conversion of linoleic acid into corresponding hydroperoxides. Inhibition of LOX by β-carotene was predominantly of the noncompetitive type. The mean K m(app) value was estimated to be 13.25μM ±0.15μM for linoleic acid while the mean K i value was estimated to be 2.56μM ±0.14μM for β-carotene. V m(app) values decreased from 1.90ΔA 234 nm/min to 0.38ΔA 234 nm/min as the concentration of β­carotene increased. The IC50 value for β-carotene was 2.49μM±0.21μM. It is shown here that β­carotene is an efficient LOX inhibitor, effective at low-level concentration enzyme-dependent peroxidation of linoleic acid. Presence of β-carotene keeps LOX in its reduced form of Fe(II) and the oxidation of linoleic acid is prevented. Springer-Verlag, 2006 Lipoxygenase nationallicence Noncompetitive inhibition nationallicence β-carotene nationallicence Inhibition mechanism nationallicence Inhibition kinetic nationallicence Serpen Arda Department of Food Engineering, Hacettepe University, 06800, Ankara, Beytepe, Turkey aut Gökmen Vural Department of Food Engineering, Hacettepe University, 06800, Ankara, Beytepe, Turkey aut European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 743-748 1438-2377 224:6<743 2007 224 217 https://doi.org/10.1007/s00217-006-0368-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0368-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Serpen Arda Department of Food Engineering, Hacettepe University, 06800, Ankara, Beytepe, Turkey aut NATIONALLICENCE 700 1- Gökmen Vural Department of Food Engineering, Hacettepe University, 06800, Ankara, Beytepe, Turkey aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 743-748 1438-2377 224:6<743 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer