caa a22 4500 463157087 CHVBK 20180406164737.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00217-006-0371-4 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0371-4 Calcium-binding peptide derived from pepsinolytic hydrolysates of hoki ( Johnius belengerii ) frame [Elektronische Daten] [Won-Kyo Jung, Se-Kwon Kim] In order to utilize fish byproducts in a calcium supplement with high solubility, hoki (Johnius belengerii) frames composed of flesh and skeleton discarded from industrial processing were degraded by pepsin in acetic acid solution (pH 2.2). After digestion, a calcium-binding peptide was isolated from the pepsinolytic hydrolysates using a hydroxyapatite affinity chromatography. Calcium-binding assay elucidated that J. belengerii frame peptide (JFP) can solubilize a similar amount of calcium with casein phosphopeptide (CPP). In ESI-QTOF tandem mass analysis for peptide identification, the amino acid sequence of JFP showed high similarity to those of actin (NCBInr database), was identified as Val-Leu-Ser-Gly-Gly-Thr-Thr-Met-Tyr-Ala-Ser-Leu-Tyr-Ala-Glu (MW: 1,561Da). Springer-Verlag, 2006 Hoki ( J. belengerii ) frame nationallicence Pepsinolytic hydrolysis nationallicence Acetic acid nationallicence Calcium-binding peptide nationallicence Jung Won-Kyo Department of Chemistry, Pukyong National University, 608-737, Busan, South Korea aut Kim Se-Kwon Department of Chemistry, Pukyong National University, 608-737, Busan, South Korea aut European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 763-767 1438-2377 224:6<763 2007 224 217 https://doi.org/10.1007/s00217-006-0371-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0371-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jung Won-Kyo Department of Chemistry, Pukyong National University, 608-737, Busan, South Korea aut NATIONALLICENCE 700 1- Kim Se-Kwon Department of Chemistry, Pukyong National University, 608-737, Busan, South Korea aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 763-767 1438-2377 224:6<763 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer