caa a22 4500 463157095 CHVBK 20180406164737.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00217-006-0373-2 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0373-2 Quantitation of type B-trichothecene mycotoxins in foods and feeds by a multiple stable isotope dilution assay [Elektronische Daten] [Stefan Asam, Michael Rychlik] For quantitation of the trichothecene mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol and 4-acetylnivalenol (Fusarenon X), stable isotope dilution assays (SIDAs) were developed by using analogues labelled with carbon-13 as the internal standards. The SIDAs presented here are the first ones reported for type B-trichothecenes. The synthesis of labelled acetyl derivatives was accomplished by [13C2]-acetylation of unlabelled trichothecenes and subsequent hydrolysis of the products. The mycotoxins were quantified in foods by LC tandem MS after cleanup on multifunctional columns. The method revealed an excellent sensitivity with low detection and quantification limits for all mycotoxins in the low μg/kg range, and good precision in inter-assay stu- dies. The analysis of a certified reference material (maize flour) resulted in a low bias of 2.1% from the certified value for DON and verified excellent trueness of the new method. To prove the suitability of SIDA, a number of cereals and cereal products were analysed and revealed DON contents ranging from 2 to 300μg/kg. Acetylated derivatives occurred in lower contents and mainly in corn products. Springer-Verlag, 2006 3-Acetyldeoxynivalenol nationallicence 15-Acetyldeoxynivalenol nationallicence Deoxynivalenol nationallicence Fusarenon X nationallicence LC-MS/MS nationallicence Stable isotope dilution assay nationallicence Trichothecenes nationallicence Asam Stefan Lehrstuhl für Lebensmittelchemie der Technischen, Universität München, Lichtenbergstr. 4, 85748, Garching, Germany aut Rychlik Michael Lehrstuhl für Lebensmittelchemie der Technischen, Universität München, Lichtenbergstr. 4, 85748, Garching, Germany aut European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 769-783 1438-2377 224:6<769 2007 224 217 https://doi.org/10.1007/s00217-006-0373-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0373-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asam Stefan Lehrstuhl für Lebensmittelchemie der Technischen, Universität München, Lichtenbergstr. 4, 85748, Garching, Germany aut NATIONALLICENCE 700 1- Rychlik Michael Lehrstuhl für Lebensmittelchemie der Technischen, Universität München, Lichtenbergstr. 4, 85748, Garching, Germany aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 769-783 1438-2377 224:6<769 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer