caa a22 4500 463157125 CHVBK 20180406164737.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00217-006-0366-1 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0366-1 Antioxidant activity of effluents during the purification of hydroxytyrosol and 3,4-dihydroxyphenyl glycol from olive oil waste [Elektronische Daten] [Guillermo Rodríguez, Rocío Rodríguez, Juan Fernández-Bolaños, Rafael Guillén, Ana Jiménez] Two different processes have been developed for the recovery of hydroxytyrosol (HT) and 3,4-dihydroxyphenyl glycol (DHPG) from olive oil mills waste. The antioxidant activity of several effluents has been characterised by four "in vitro” tests: antiradical capacity (ARC), ferric reducing power (P R) and inhibition of primary and secondary oxidation in lipid systems. HT-containing effluents exhibited higher or similar activity in ARC and P R than equimolecular quantities of vitamins E and C. In oxidative tests they were between both assayed vitamins. Except for commercial Hytolive®2, activities correlated with HT concentration of each sample. DHPG-containing effluent had higher activities than a solution with the same concentration of the synthetic DL-DHPG. This effluent was the most active in ARC and P R tests, its activity being similar to vitamin E in the inhibition of primary oxidation. Both phenolic compounds showed antagonist effect when they coeluted, except for P R assay. These results suggested that due to the low-cost procedure and to the antioxidant activity of effluents, they could be valuable as natural additives for food, pharmaceutical and cosmetic industries. Springer-Verlag, 2006 Olive oil byproduct nationallicence Hydroxytyrosol nationallicence 3,4-Dihydroxyphenyl glycol nationallicence Purification effluents nationallicence Natural antioxidants nationallicence HT : hydroxytyrosol nationallicence DHPG : 3,4-dihydroxyphenyl glycol nationallicence DPPH : 2,2-diphenyl-1-picrylhydrazyl (free radical) nationallicence ABAP : 2,2′-azobis(2-amidinopropane) dihydrochloride nationallicence SDS : sodium dodecylsulphate nationallicence RT : retention time nationallicence EC50 : amount of antioxidant necessary to decrease the initial absorbance by 50% nationallicence EC25 : amount of antioxidant necessary to decrease the initial absorbance by 25% nationallicence ARC : antiradical capacity nationallicence P R : reducing power nationallicence QE : quercetin equivalent nationallicence POIC : primary oxidation inhibition capacity nationallicence TBARS : thiobarbituric acid reactive species nationallicence R : correlation coefficient nationallicence Rodríguez Guillermo Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut Rodríguez Rocío Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut Fernández-Bolaños Juan Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut Guillén Rafael Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut Jiménez Ana Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 733-741 1438-2377 224:6<733 2007 224 217 https://doi.org/10.1007/s00217-006-0366-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0366-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodríguez Guillermo Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Rodríguez Rocío Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Fernández-Bolaños Juan Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Guillén Rafael Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut NATIONALLICENCE 700 1- Jiménez Ana Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/6(2007-04-01), 733-741 1438-2377 224:6<733 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer